Emilsson Gustav, Sakiyama Yusuke, Malekian Bita, Xiong Kunli, Adali-Kaya Zeynep, Lim Roderick Y H, Dahlin Andreas B
Department of Chemistry and Chemical Engineering, Chalmers University of Technology, 41296 Göteborg, Sweden.
Biozentrum and the Swiss Nanoscience Institute, University of Basel, 4056 Basel, Switzerland.
ACS Cent Sci. 2018 Aug 22;4(8):1007-1014. doi: 10.1021/acscentsci.8b00268. Epub 2018 Jul 26.
Control of molecular translocation through nanoscale apertures is of great interest for DNA sequencing, biomolecular filters, and new platforms for single molecule analysis. However, methods for controlling the permeability of nanopores are very limited. Here, we show how nanopores functionalized with poly(ethylene glycol) brushes, which fully prevent protein translocation, can be reversibly gated to an "open" state by binding of single IgG antibodies that disrupt the macromolecular barrier. On the basis of surface plasmon resonance data we propose a two-state model describing the antibody-polymer interaction kinetics. Reversibly (weakly) bound antibodies decrease the protein exclusion height while irreversibly (strongly) bound antibodies do not. Our results are further supported by fluorescence readout from pore arrays and high-speed atomic force microscopy on single pores. This type of dynamic barrier control on the nanoscale provides new possibilities for biomolecular separation and analysis.
通过纳米级孔径控制分子转运对于DNA测序、生物分子过滤器以及单分子分析的新平台而言极具意义。然而，控制纳米孔渗透性的方法非常有限。在此，我们展示了用聚乙二醇刷功能化的纳米孔，这种纳米孔能完全阻止蛋白质转运，通过破坏大分子屏障的单IgG抗体结合，可将其可逆地门控到“开放”状态。基于表面等离子体共振数据，我们提出了一个描述抗体 - 聚合物相互作用动力学的双态模型。可逆（弱）结合的抗体降低了蛋白质排斥高度，而不可逆（强）结合的抗体则不会。我们的结果进一步得到了孔阵列荧光读数以及单孔高速原子力显微镜的支持。这种纳米级的动态屏障控制为生物分子分离和分析提供了新的可能性。
