甾体研究。CCXIX。高效液相色谱-电化学检测法分离和测定生物体液中的4-羟基雌三醇单葡萄糖醛酸苷和单硫酸盐。
Studies on steroids. CCXIX. Separation and determination of 4-hydroxyoestriol monoglucuronides and monosulphates in biological fluids by high-performance liquid chromatography with electrochemical detection.
作者信息
Shimada K, Xie F M, Nambara T
出版信息
J Chromatogr. 1986 May 28;378(1):17-24.
The separation and determination of 4-hydroxyoestriol monoglucuronides and monosulphates by high-performance liquid chromatography with electrochemical detection on a reversed-phase column has been carried out. The effects of the salt, composition and pH of the mobile phase on the resolution were investigated with a Develosil ODS-5 column. Each group of isomeric monoglucuronides and monosulphates of 4-hydroxyoestriol was efficiently resolved on this column when 0.5% sodium acetate-acetonitrile and 0.5% sodium acetate-tetrahydrofuran-acetonitrile were used as mobile phases, respectively. The use of the present method revealed that 4-hydroxyoestriol orally administered to the rat was excreted as 4-, 3-, 16-glucuronides and 4-sulphate in bile.
采用反相柱高效液相色谱-电化学检测法对4-羟基雌三醇单葡萄糖醛酸苷和单硫酸盐进行了分离和测定。使用Develosil ODS-5柱研究了流动相的盐、组成和pH对分离度的影响。当分别使用0.5%醋酸钠-乙腈和0.5%醋酸钠-四氢呋喃-乙腈作为流动相时，4-羟基雌三醇的每组异构单葡萄糖醛酸苷和单硫酸盐在该柱上均能有效分离。本方法的应用表明，给大鼠口服4-羟基雌三醇后，其在胆汁中以4-、3-、16-葡萄糖醛酸苷和4-硫酸盐的形式排泄。
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