通过高压液相色谱法分离雌激素共轭物。

Separation of estrogen conjugates by high pressure liquid chromatography.

作者信息

Musey P I, Collins D C, Preedy J R

出版信息

Steroids. 1978 Apr;31(4):583-92. doi: 10.1016/0039-128x(78)90039-9.

PMID:208201

Abstract

High pressure liquid chromatography using a prepacked commercial strong anion exchanger column (mu Partisil 10 SAX, 25 cm x 4.6 mm) was used to separate a mixture of eight estrogen conjugates. Chromatographic conditions using a 0.01 M potassium phosphate or 0.1 M NaCl as solvent in the isocratic mode are described for the separation of estrone glucosiduronate, 17beta-estradiol-3-glucosiduronate, 17beta-estradiol-17-glucosiduronate, estriol-3-glucosiduronate, estriol-16alpha-glucosiduronate, estriol-17-glucosiduronate, estrone sulfate and 17beta-estradiol-3-sulfate. This system gives high resolution of the estrogen conjugates in small eluent volumes in less than 30 min. The advantages of this high pressure liquid chromatographic system over other methods of separation are discussed.

摘要

使用预填充的商用强阴离子交换柱（μ Partisil 10 SAX，25 cm×4.6 mm）的高压液相色谱法用于分离八种雌激素结合物的混合物。描述了在等度模式下使用0.01 M磷酸钾或0.1 M氯化钠作为溶剂的色谱条件，用于分离雌酮葡萄糖醛酸苷、17β - 雌二醇 - 3 - 葡萄糖醛酸苷、17β - 雌二醇 - 17 - 葡萄糖醛酸苷、雌三醇 - 3 - 葡萄糖醛酸苷、雌三醇 - 16α - 葡萄糖醛酸苷、雌三醇 - 17 - 葡萄糖醛酸苷、雌酮硫酸盐和17β - 雌二醇 - 3 - 硫酸盐。该系统在不到30分钟的时间内，以少量洗脱剂即可实现雌激素结合物的高分辨率分离。讨论了该高压液相色谱系统相对于其他分离方法的优势。