从苏丹看似健康的牛只血清学调查采集的血清中分离出蓝舌病毒血清群。
Recovery of bluetongue virus serogroup from sera collected for a serological survey from apparently healthy cattle, from the Sudan.
作者信息
Abu Elzein E M
出版信息
J Hyg (Lond). 1986 Jun;96(3):529-33. doi: 10.1017/s002217240006633x.
Virus of the bluetongue (BT) serogroup was recovered from 11% of cattle sera collected from apparently healthy animals in Khartoum Province for the sole purpose of screening for BT antibodies. Since these sera did not contain BT antibodies, the donor cattle could have been scored as BT free in the serological survey. Virus was initially isolated in chicken embryos inoculated intravascularly, and was further adapted to Vero cell cultures. Isolates were identified as belonging to the BT serogroup using the agar gel immunodiffusion (AGID) and complement fixation (CF) tests. The results indicated that cattle in the Sudan could harbour BT virus without showing symptoms of the disease. Such an observation necessitates further work to clarify the role of cattle in the epidemiology of BT in the Sudan.
从喀土穆省表面健康的动物采集的牛血清中，有11%分离出了蓝舌病（BT）血清群病毒，采集这些血清的唯一目的是筛查BT抗体。由于这些血清不含BT抗体，在血清学调查中这些供体牛本可被判定为无BT。病毒最初是通过血管内接种在鸡胚中分离出来的，之后进一步适应了Vero细胞培养。使用琼脂凝胶免疫扩散（AGID）和补体结合（CF）试验将分离株鉴定为属于BT血清群。结果表明，苏丹的牛可能携带BT病毒而不表现出疾病症状。这一观察结果需要进一步开展工作，以阐明牛在苏丹BT流行病学中的作用。
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