脊髓灰质炎病毒中和作用与聚集之间的关系。
Relationship between poliovirus neutralization and aggregation.
作者信息
Thomas A A, Vrijsen R, Boeyé A
出版信息
J Virol. 1986 Aug;59(2):479-85. doi: 10.1128/JVI.59.2.479-485.1986.
Abstract
The interaction of mono- and polyclonal neutralizing antibodies with poliovirus was studied. In all cases, neutralization was due to antibody-mediated virus aggregation, and the unpolymerized virions accounted for the residual infectivity. The effect of papain on previously neutralized virus was to deaggregate the virus to fully infective single virions. With some antibodies, the amount of aggregated virus regressed in the region of greatest antibody excess, even though the virus remained fully neutralized. Under these conditions, noninfective, unaggregated immune complexes were formed. A mutant resistant to one of the monoclonal antibodies was selected. The mutant virions were still bound but no longer aggregated or neutralized by the selecting antibodies.
摘要
研究了单克隆和多克隆中和抗体与脊髓灰质炎病毒的相互作用。在所有情况下，中和作用均归因于抗体介导的病毒聚集，未聚合的病毒粒子导致了残余感染性。木瓜蛋白酶对先前已中和病毒的作用是使病毒解聚为完全具有感染性的单个病毒粒子。对于某些抗体，即使病毒仍被完全中和，在抗体过量最多的区域聚集病毒的量仍会减少。在这些条件下，形成了无感染性的、未聚集的免疫复合物。筛选出了一种对其中一种单克隆抗体具有抗性的突变体。突变病毒粒子仍能结合，但不再被筛选抗体聚集或中和。
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