Peters G, Placzek M, Brookes S, Kozak C, Smith R, Dickson C
J Virol. 1986 Sep;59(3):535-44. doi: 10.1128/JVI.59.3.535-544.1986.
In the course of analyzing sites of proviral integration in tumors induced by mouse mammary tumor virus (MMTV), we have isolated recombinant DNA clones corresponding to the 5' and 3' ends of four endogenous MMTV proviruses present in BALB/c and BR6 mice. This has permitted the structural characterization of each locus by detailed restriction mapping and the preparation of DNA probes specific for the cellular sequences flanking each provirus. These probes have been used to trace the segregation patterns of the proviruses, designated Mtv-8, Mtv-9, Mtv-17, and Mtv-21, in a panel of inbred strains of laboratory mice and to map Mtv-17 and Mtv-21 to mouse chromosomes 4 and 8, respectively. The unambiguous resolution of these four proviruses on Southern blots has greatly facilitated the analysis of other endogenous MMTV proviruses in these inbred mice.
在分析小鼠乳腺肿瘤病毒（MMTV）诱导的肿瘤中前病毒整合位点的过程中，我们分离出了与BALB/c和BR6小鼠中存在的四种内源性MMTV前病毒的5'和3'末端相对应的重组DNA克隆。这使得通过详细的限制性图谱分析每个位点的结构特征，并制备针对每个前病毒侧翼细胞序列的DNA探针成为可能。这些探针已被用于追踪在一组近交系实验小鼠中指定为Mtv-8、Mtv-9、Mtv-17和Mtv-21的前病毒的分离模式，并分别将Mtv-17和Mtv-21定位到小鼠4号和8号染色体上。这四种前病毒在Southern印迹上的明确分辨极大地促进了对这些近交小鼠中其他内源性MMTV前病毒的分析。
