Steele P E, Martin M A, Rabson A B, Bryan T, O'Brien S J
J Virol. 1986 Sep;59(3):545-50. doi: 10.1128/JVI.59.3.545-550.1986.
Endogenous retroviral sequences in humans have undergone amplification events involving both viral and flanking cellular sequences. We cloned members of an amplified family of full-length endogenous retroviral sequences. Genomic blotting, employing a flanking cellular DNA probe derived from a member of this family, revealed a similar array of reactive bands in both humans and chimpanzees, indicating that an amplification event involving retroviral and associated cellular DNA sequences occurred before the evolutionary separation of these two primates. Southern analyses of restricted somatic cell hybrid DNA preparations suggested that endogenous retroviral segments are widely dispersed in the human genome and that amplification and dispersion events may be linked.
人类内源性逆转录病毒序列经历了涉及病毒和侧翼细胞序列的扩增事件。我们克隆了一个全长内源性逆转录病毒序列扩增家族的成员。使用源自该家族成员的侧翼细胞DNA探针进行基因组印迹分析，结果显示人类和黑猩猩中出现了相似的反应带阵列，这表明涉及逆转录病毒和相关细胞DNA序列的扩增事件发生在这两种灵长类动物进化分离之前。对限制性体细胞杂种DNA制备物的Southern分析表明，内源性逆转录病毒片段在人类基因组中广泛分布，并且扩增和分散事件可能相关联。
