Tarantul V Z, Kucheriavy V V, Makarova I V, Begetova T V, Andreeva L E, Gazaryan K G
Mol Gen Genet. 1986 May;203(2):305-11. doi: 10.1007/BF00333971.
Previously, mouse zygotes were microinjected with the recombinant plasmid pMA3, which contains the Herpes simplex virus thymidine kinase gene attached to the promoter region of the Rous sarcoma virus (Gazaryan et al. 1984a). In the present work the pMA3 fragment with the flanking genomic sequences was isolated from the DNA of one transgenic Fo mouse by the "plasmid rescue" technique. The rescued plasmid (pMAR1) lacked all virus-specific sequences and retained only some pBR322 sequences. The flanking region at one end of the integrated pBR322-specific fragment contained a highly conserved mouse repetitive sequence. The possible mechanisms of rearrangement of foreign DNA in germ line cells are discussed.
此前，已将重组质粒pMA3显微注射到小鼠受精卵中，该质粒含有与劳氏肉瘤病毒启动子区域相连的单纯疱疹病毒胸苷激酶基因（加扎良等人，1984年a）。在本研究中，通过“质粒拯救”技术从一只转基因F0小鼠的DNA中分离出带有侧翼基因组序列的pMA3片段。拯救出的质粒（pMAR1）缺少所有病毒特异性序列，仅保留了一些pBR322序列。整合的pBR322特异性片段一端的侧翼区域包含一个高度保守的小鼠重复序列。本文讨论了种系细胞中外源DNA重排的可能机制。
