Eilers M, Schatz G
Nature. 1986;322(6076):228-32. doi: 10.1038/322228a0.
Methotrexate, a folate antagonist, blocks import into mitochondria of mouse dihydrofolate reductase fused to a mitochondrial presequence. Methotrexate does not mask the presequence, but stabilizes the dihydrofolate reductase moiety. It does not inhibit import of the authentic precursor from which the presequence is derived. This suggests that dihydrofolate reductase must at least partly unfold in order to be transported across mitochondrial membranes.
甲氨蝶呤是一种叶酸拮抗剂，它能阻断与线粒体前导序列融合的小鼠二氢叶酸还原酶进入线粒体。甲氨蝶呤不会掩盖前导序列，但会稳定二氢叶酸还原酶部分。它不会抑制前导序列所源自的真实前体的导入。这表明二氢叶酸还原酶必须至少部分展开才能穿过线粒体膜进行转运。
