Pain D, Murakami H, Blobel G
Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.
Nature. 1990 Oct 4;347(6292):444-9. doi: 10.1038/347444a0.
Anti-idiotypic antibodies, prepared using a chemically synthesized signal peptide of a mitochondrial precursor protein, recognized a mitochondrial integral membrane protein (p32). Fab fragments derived from both anti-idiotypic antibodies and monospecific antibodies against purified p32 inhibited protein import into mitochondria. Moreover, anti-p32 antibodies specifically immunoprecipitated a precursor-p32 complex after detergent solubilization of mitochondria. Immunoelectron microscopy and subfractionation of mitochondria indicate that p32 is located in contact sites between the outer and inner mitochondrial membranes.
使用线粒体前体蛋白的化学合成信号肽制备的抗独特型抗体识别出线粒体整合膜蛋白（p32）。源自抗独特型抗体和针对纯化的p32的单特异性抗体的Fab片段均抑制蛋白质导入线粒体。此外，抗p32抗体在去污剂溶解线粒体后特异性免疫沉淀前体-p32复合物。免疫电子显微镜和线粒体亚分级分离表明p32位于线粒体外膜和内膜之间的接触位点。
