1 Department of Orthopedics, UHealth Sports Medicine Institute, University of Miami Miller School of Medicine, Miami, Florida.
2 Diabetes Research Institute & Cell Transplant Center, University of Miami Miller School of Medicine, Miami, Florida.
Tissue Eng Part B Rev. 2019 Feb;25(1):55-77. doi: 10.1089/ten.TEB.2018.0118. Epub 2018 Oct 27.
Culture expansion of MSCs has detrimental effects on various cell characteristics and attributes (e.g., phenotypic changes and senescence), which, in addition to inherent interdonor variability, negatively impact the standardization and reproducibility of their therapeutic potential. The identification of innate distinct functional MSC subpopulations, as well as the description of ex vivo protocols aimed at maintaining phenotypes and enhancing specific functions have the potential to overcome these limitations. The incorporation of those approaches into cell-based therapy would significantly impact the field, as more reproducible clinical outcomes may be achieved.
间充质干细胞的培养会对各种细胞特性和属性（例如表型改变和衰老）产生有害影响，除了固有供体间的变异性外，这也会对其治疗潜力的标准化和可重复性产生负面影响。鉴定固有不同功能的间充质干细胞亚群，以及描述旨在维持表型和增强特定功能的体外方案，有可能克服这些限制。将这些方法纳入基于细胞的治疗中会对该领域产生重大影响，因为可能会获得更可重复的临床结果。
