Kotelnikova P A, Shipunova V O, Aghayeva U F, Stremovskiy O A, Nikitin M P, Novikov I A, Schulga A A, Deyev S M, Petrov R V
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Moscow oblast, Russia.
Dokl Biochem Biophys. 2018 Jul;481(1):198-200. doi: 10.1134/S1607672918040051. Epub 2018 Aug 31.
A new method for obtaining biomodified magnetite nanoparticles for targeted delivery to cells was developed. The method is based on the use of the C-terminal fragment of the Mms6 protein, which is involved in the magnetite biomineralization during the synthesis of magnetosomes in magnetotactic bacteria Magnetospirillum magneticum AMB-1, and the barnasebarstar high-affinity protein pair. The Mms6 protein fragment is required for stabilizing magnetite, and the barnasebarstar pair mediates the interaction between nanoparticles and the component for modification. The efficiency of this method was confirmed in the synthesis of magnetite nanoparticles recognizing the HER2/neu tumor marker and in the selective labeling of HER2/neu with these nanoparticles on the surface of cancer cells.
开发了一种获取生物改性磁铁矿纳米颗粒以靶向递送至细胞的新方法。该方法基于使用Mms6蛋白的C末端片段，该片段在趋磁细菌Magnetospirillum magneticum AMB-1中磁小体合成过程中参与磁铁矿生物矿化，以及芽孢杆菌RNA酶芽孢杆菌抗酶高亲和力蛋白对。Mms6蛋白片段是稳定磁铁矿所必需的，而芽孢杆菌RNA酶芽孢杆菌抗酶对介导纳米颗粒与修饰成分之间的相互作用。该方法的有效性在识别HER2/neu肿瘤标志物的磁铁矿纳米颗粒合成以及用这些纳米颗粒在癌细胞表面对HER2/neu进行选择性标记中得到了证实。
