Stevenson M, Volsky B, Hedenskog M, Volsky D J
Science. 1986 Aug 29;233(4767):980-4. doi: 10.1126/science.3016899.
Epstein-Barr virus (EBV), a ubiquitous human herpesvirus, has the ability to transform human B lymphocytes. No other cell type has been experimentally transformed by EBV, either by intact virions or naked viral DNA and subgenomic fragments. Two immortalized human T-lymphoblastoid cell lines have now been established by transfecting cord blood lymphocytes with purified B95-8 viral DNA enclosed in fusogenic Sendai virus envelopes (RSVE) and then exposing the cells to EBV from a P3HR-1 cell subclone. One of these lines, which has been fully characterized, is termed HBD-1. This line is positive for EBV DNA and expresses surface OKT11, OKT4, and Tac receptors, but not M-1, mu immunoglobulin chains, EBV receptors, or B-1 surface markers. The cells contain fully rearranged T-cell receptor genes and germline immunoglobulin genes. The karyotype of the cells is normal, they do not require interleukin-2 for growth, and do not contain human T-lymphotropic virus type I. However, the HBD-1 cells contain incomplete EBV genomes and express several EBV-determined antigens, including the early antigen type D, membrane antigens, but not EBV-determined nuclear antigen (EBNA). This association of the EBV genome with permanently growing hematopoietic cells of non B-cell lineage should prove useful in studies on the mechanism of EBV-mediated cell transformation.
爱泼斯坦-巴尔病毒（EBV）是一种普遍存在的人类疱疹病毒，具有转化人类B淋巴细胞的能力。无论是完整的病毒体还是裸露的病毒DNA及亚基因组片段，都未通过实验使其他细胞类型被EBV转化。现在，通过用包裹在融合性仙台病毒包膜（RSVE）中的纯化B95-8病毒DNA转染脐血淋巴细胞，然后将这些细胞暴露于来自P3HR-1细胞亚克隆的EBV，已建立了两个永生化的人类T淋巴母细胞系。其中一个已被充分鉴定的系被称为HBD-1。该系EBV DNA呈阳性，表达表面OKT11、OKT4和Tac受体，但不表达M-1、μ免疫球蛋白链、EBV受体或B-1表面标志物。这些细胞含有完全重排的T细胞受体基因和种系免疫球蛋白基因。细胞的核型正常，生长不需要白细胞介素-2，也不含有I型人类嗜T淋巴细胞病毒。然而，HBD-1细胞含有不完整的EBV基因组，表达几种EBV决定的抗原，包括D型早期抗原、膜抗原，但不表达EBV决定的核抗原（EBNA）。EBV基因组与非B细胞系的永久生长造血细胞的这种关联在研究EBV介导的细胞转化机制中应会证明是有用的。
