溶解气体对超声化学和生物学效应的影响。

Influence of dissolved gases on chemical and biological effects of ultrasound.

作者信息

Kondo T, Kuwabara M, Sato F, Kano E

出版信息

Ultrasound Med Biol. 1986 Feb;12(2):151-5. doi: 10.1016/0301-5629(86)90020-7.

Abstract

The influence of dissolved gases (O2, Ar, N2O, and CO2) on the chemical and biological effects of 1.2 MHz continuous wave ultrasound was investigated. Spin-trapping of OH and H radicals with 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) and observation of iodine liberation from a potassium iodide-starch solution were employed for monitoring the chemical effects, while lysing of mouse L5178Y cells was employed for monitoring the biological effects. The effectiveness of the dissolved gases in producing OH-DMPO adducts and H-DMPO adducts was O2 greater than Ar greater than N2O = CO2 approximately equal to O and Ar greater than O2 = N2O = CO2 approximately equal to O, respectively. A result similar to the yield of OH-DMPO was obtained from the liberation of iodine induced by ultrasound. In addition, the effectiveness of the dissolved gases in lysing mouse L5178Y cells by ultrasound was O2 = Ar greater than N2O = CO2 approximately equal to O. These results suggest that both dissolved N2O and CO2 gases in solution suppressed not only the chemical effect but also the biological effect.

摘要

研究了溶解气体（O₂、Ar、N₂O和CO₂）对1.2 MHz连续波超声的化学和生物学效应的影响。采用5,5-二甲基-1-吡咯啉N-氧化物（DMPO）对OH和H自由基进行自旋捕获，并观察碘化钾-淀粉溶液中碘的释放来监测化学效应，同时采用小鼠L5178Y细胞裂解来监测生物学效应。溶解气体产生OH-DMPO加合物和H-DMPO加合物的有效性分别为O₂大于Ar大于N₂O = CO₂约等于O，以及Ar大于O₂ = N₂O = CO₂约等于O。超声诱导碘释放得到了与OH-DMPO产率相似的结果。此外，溶解气体通过超声裂解小鼠L5178Y细胞的有效性为O₂ = Ar大于N₂O = CO₂约等于O。这些结果表明，溶液中溶解的N₂O和CO₂气体不仅抑制了化学效应，还抑制了生物学效应。