Faelen M, Toussaint A, Waggoner B, Desmet L, Pato M
Virology. 1986 Aug;153(1):70-9. doi: 10.1016/0042-6822(86)90008-5.
The insertion of DNA fragments within the lac sequence of a MudI(Ap,lac) prophage resulted in the formation of a set of maxi-Mu genomes which were 39.8, 59, 85.6, and 88.2 kb long, respectively. The comparison of these maxi-Mu's with the 37.2-kb-long parental MudI(Ap,lac) indicated that the transposition frequency decreased as the length of the prophage increased. No replication of the two longest maxi-Mu's could be detected. The 59- and the 39.8-kb-long chimeric genomes were noted to replicate at approximately 1-2 and 30%, respectively, of the rate found with the MudI(Ap,lac) prophage. The length dependence of the transposition and replication could be explained by the impairment of an early step of the transposition/replication mechanism.
将DNA片段插入MudI(Ap,lac)原噬菌体的lac序列内，导致形成了一组最大Mu基因组，其长度分别为39.8、59、85.6和88.2 kb。将这些最大Mu与长度为37.2 kb的亲本MudI(Ap,lac)进行比较，结果表明转座频率随着原噬菌体长度的增加而降低。未检测到两个最长的最大Mu的复制。注意到长度为59 kb和39.8 kb的嵌合基因组的复制率分别约为MudI(Ap,lac)原噬菌体复制率的1-2%和30%。转座和复制的长度依赖性可以通过转座/复制机制早期步骤的损伤来解释。
