Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information (FASC), Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium; Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling (FABI), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information (FASC), Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
J Pharm Biomed Anal. 2018 Nov 30;161:192-205. doi: 10.1016/j.jpba.2018.08.043. Epub 2018 Aug 23.
In vivo determination of brain mediators plays an important role in providing insight in how the brain functions. For this purpose, targeted metabolomics can be a very useful tool. Targeted metabolomics detects and measures certain known low-molecular-weight biomolecules involved in signaling pathways and biochemical processes in the central nervous system. Microdialysis is a powerful technique to sample brain mediators in the central nervous system. Several analytical techniques that can possibly be coupled to microdialysis are available. However, selection of an appropriate technique should be considered carefully, since sensitivity and specificity are critical when measuring these mediators in volume-restricted microdialysis samples. This review outlines some of the commonly applied sampling methods and analytical techniques and discusses some of the challenges encountered during the in vivo determination of central nervous system mediators.
在体脑内介质的测定对于了解大脑功能具有重要作用。为此，靶向代谢组学可能是一种非常有用的工具。靶向代谢组学检测和测量特定的已知低分子量生物分子，这些生物分子参与中枢神经系统中的信号通路和生化过程。微透析是一种从中枢神经系统中采样脑内介质的强大技术。有几种可能与微透析相结合的分析技术。然而，在测量这些在体积受限的微透析样本中测量时，应仔细考虑选择合适的技术，因为灵敏度和特异性至关重要。本综述概述了一些常用的采样方法和分析技术，并讨论了在体内测定中枢神经系统介质时遇到的一些挑战。
