雅培人嗜T淋巴细胞病毒III型酶免疫测定法（一种检测供血者血液中抗人嗜T淋巴细胞病毒III型抗体的检测方法）的性能评估

Performance evaluation of the Abbott HTLV III EIA, a test for antibody to HTLV III in donor blood.

作者信息

Barrett J E, Dawson G, Heller J, Bairstow C, Fico R, Webber J S, Gutierrez R, Decker R H

出版信息

Am J Clin Pathol. 1986 Aug;86(2):180-5. doi: 10.1093/ajcp/86.2.180.

DOI:10.1093/ajcp/86.2.180

Abstract

An enzyme immunoassay (EIA), designed to detect antibodies to human T-cell lymphotropic virus type III (HTLV III), was evaluated. The antibody test was found to be highly sensitive; serum from 221 of 223 (99.1%) patients with acquired immune deficiency syndrome (AIDS) was positive for antibodies to HTLV III. In addition, the antibody test was found to be highly specific; approximately 99.75% of 20,720 serum or plasma samples from blood donors were negative for antibody to HTLV III. In most cases, the Western Blot analysis agreed well with the EIA. Eighty-one of 82 (98.8%) EIA-positive samples from patients with AIDS were Western Blot positive. Of the EIA-positive blood donors, 21 of 36 (58%) were detected and confirmed by Western Blot analysis. A solid-phase competitive EIA also was evaluated as an alternate procedure. The preliminary results indicate that this immunoassay has a high degree of sensitivity and specificity and could serve as an alternate procedure to detect antibody to HTLV III.

摘要

对一种旨在检测抗人类III型嗜T细胞病毒（HTLV III）抗体的酶免疫测定法（EIA）进行了评估。发现该抗体检测具有高度敏感性；223例获得性免疫缺陷综合征（AIDS）患者中有221例（99.1%）的血清抗HTLV III抗体呈阳性。此外，还发现该抗体检测具有高度特异性；20720份来自献血者的血清或血浆样本中约99.75%抗HTLV III抗体呈阴性。在大多数情况下，蛋白质印迹分析与EIA结果高度一致。82例AIDS患者的EIA阳性样本中有81例（98.8%）蛋白质印迹呈阳性。在EIA阳性的献血者中，36例中有21例（58%）经蛋白质印迹分析检测并确认。还对一种固相竞争性EIA作为替代方法进行了评估。初步结果表明，这种免疫测定法具有高度的敏感性和特异性，可作为检测抗HTLV III抗体的替代方法。