Regensdorff Merle, Deckena Marek, Stein Maike, Borchers Agnes, Scherer Gwydion, Lammers Maike, Hänsch Robert, Zachgo Sabine, Buschmann Henrik
Botany Department, School of Biology and Chemistry, Osnabrück University, Barbarastraße 11, 49076, Osnabrück, Germany.
Department of Plant Biology, Technische Universität Braunschweig, Humboldtstraße 1, 38106, Braunschweig, Germany.
J Phycol. 2018 Dec;54(6):840-849. doi: 10.1111/jpy.12781. Epub 2018 Sep 21.
Mougeotia scalaris is a filamentous streptophyte alga renowned for light-inducible plastid rotation and microtubule-dependent polarity establishment. As a first step toward transgenic approaches we determined the 5,825 base pair genomic sequence encoding the α-tubulin1 gene (MsTUA1) of M. scalaris (strain SAG 164.80). The subcloned MsTUA1 promoter facilitated strong transgene expression in M. scalaris and tobacco leaf cells, as shown by particle bombardment and the subsequent visualization of expressed fluorescent protein markers. Our results provide a route for the genetic transformation of the filamentous streptophyte alga M. scalaris based on the endogenous TUA1 promoter.
标桩转板藻是一种丝状链形植物藻类，以光诱导质体旋转和微管依赖的极性建立而闻名。作为转基因方法的第一步，我们确定了编码标桩转板藻（菌株SAG 164.80）α-微管蛋白1基因（MsTUA1）的5825个碱基对的基因组序列。如通过粒子轰击以及随后对表达的荧光蛋白标记的可视化所示，亚克隆的MsTUA1启动子促进了标桩转板藻和烟草叶细胞中强转基因表达。我们的结果为基于内源性TUA1启动子的丝状链形植物藻类标桩转板藻的遗传转化提供了一条途径。
