niChe Lab for Stem Cell and Regenerative Medicine, Department of Biochemical Science and Technology, National Taiwan University, Taiwan; Department of Pathology, Wan Fang Hospital, Taipei Medical University, Taiwan.
niChe Lab for Stem Cell and Regenerative Medicine, Department of Biochemical Science and Technology, National Taiwan University, Taiwan.
J Formos Med Assoc. 2018 Dec;117(12):1046-1057. doi: 10.1016/j.jfma.2018.06.013. Epub 2018 Aug 29.
Induced pluripotent stem cells (iPSCs) are embryonic stem cell-like cells reprogrammed from somatic cells by four transcription factors, OCT4, SOX2, KLF4 and c-MYC. iPSCs derived from cancer cells (cancer-iPSCs) could be a novel strategy for studying cancer. During cancer cell reprogramming, the epigenetic status of the cancer cell may be altered, such that it acquires stemness and pluripotency. The cellular behavior of the reprogrammed cells exhibits dynamic changes during the different stages of reprogramming. The cells may acquire the properties of cancer stem cells (CSCs) during the process of reprogramming, and lose their carcinogenic properties during reprogramming into a cancer-iPSCs. Differentiation of cancer-iPSCs by teratoma formation or organoid culturing could mimic the process of tumorigenesis. Some of the molecular mechanisms associated with cancer progression could be elucidated using the cancer-iPSC model. Furthermore, cancer-iPSCs could be expanded in culture system or bioreactors, and serve as cell sources for research, and as personal disease models for therapy and drug screening. This article introduces cancer studies that used the cell reprogramming strategy.
诱导多能干细胞（iPSCs）是通过四个转录因子（OCT4、SOX2、KLF4 和 c-MYC）将体细胞重编程为胚胎干细胞样细胞。源自癌细胞的 iPSCs（癌症-iPSCs）可能是研究癌症的一种新策略。在癌细胞重编程过程中，癌细胞的表观遗传状态可能会发生改变，从而获得干性和多能性。在不同的重编程阶段，重编程细胞的细胞行为会发生动态变化。在重编程过程中，这些细胞可能获得癌症干细胞（CSCs）的特性，并在重编程为癌症-iPSCs 的过程中失去致癌特性。通过畸胎瘤形成或类器官培养使癌症-iPSCs 分化，可以模拟肿瘤发生的过程。可以使用癌症-iPSC 模型阐明与癌症进展相关的一些分子机制。此外，癌症-iPSCs 可以在培养系统或生物反应器中扩增，并作为研究的细胞来源，以及作为个人疾病模型进行治疗和药物筛选。本文介绍了使用细胞重编程策略进行的癌症研究。
