Kanaji K, Okuma M, Sugiyama T, Sensaki S, Ushikubi F, Uchino H
Biochem Biophys Res Commun. 1986 Jul 31;138(2):589-95. doi: 10.1016/s0006-291x(86)80537-x.
Stimulation of human neutrophils with 12-hydroperoxyeicosatetraenoic acid (12-HPETE) led to formation of 5S, 12S-dihydroxyeicosatetraenoic acid (DiHETE), but leukotriene B4 (LTB4) or 5-hydroxyeicosatetraenoic acid (5-HETE) was not detectable by reversed-phase high-performance liquid chromatography analysis. N-formylmethionylleucylphenylalanine (FMLP) induced the additional synthesis of small amounts of LTB4 in 12-HPETE-stimulated neutrophils. The addition of arachidonic acid greatly increased the synthesis of LTB4 and 5-HETE by neutrophils incubated with 12-HPETE. In experiments using [1-14C]arachidonate-labeled neutrophils, little radioactivity was released by 12-HPETE alone or by 12-HPETE plus FMLP, while several radiolabeled compounds, including LTB4 and 5-HETE, were released by A23187. These findings demonstrate that LTB4 biosynthesis by 12-HPETE-stimulated neutrophils requires free arachidonic acid which may be endogenous or exogenous.
用12-氢过氧化二十碳四烯酸（12-HPETE）刺激人中性粒细胞可导致5S，12S-二羟基二十碳四烯酸（DiHETE）的形成，但通过反相高效液相色谱分析未检测到白三烯B4（LTB4）或5-羟基二十碳四烯酸（5-HETE）。N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）在12-HPETE刺激的中性粒细胞中诱导少量LTB4的额外合成。添加花生四烯酸极大地增加了与12-HPETE一起孵育的中性粒细胞中LTB4和5-HETE的合成。在使用[1-14C]花生四烯酸盐标记的中性粒细胞的实验中，单独的12-HPETE或12-HPETE加FMLP释放的放射性很少，而包括LTB4和5-HETE在内的几种放射性标记化合物由A23187释放。这些发现表明，12-HPETE刺激的中性粒细胞合成LTB4需要游离的花生四烯酸，其可以是内源性的或外源性的。
