Guo Jun, Yan Bin, Wang Fu, Gao Qing-He, Zhang Xiu-Ju, Yu Guo-Jin, Zeng Yin, Qiu Jun-Feng, Geng Qiang, Han Qiang
Department of Andrology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
Department of Andrology, The First Affiliated Hospital of Tianjin University of Chinese Medicine, Tianjin 300192, China.
Zhonghua Nan Ke Xue. 2018 Aug;24(8):724-728.
To observe the intervention effect of Qiaoshao Prescription (QSP) on premature ejaculation (PE) induced by 8-OH-DPAT in male rats and explore its possible action mechanism.
Seventy-two male Wistar rats were equally randomized into six groups, blank control, PE model control, low-, medium- and high-dose QSP, and dapoxetine. The PE model was established by injection of 8-OH-DPAT into the subarachnoid space of the lumbosacral spinal cord. Four weeks after modeling, the rats in the blank control and PE model control groups with gavaged with normal saline at 10 ml/kg/d, those in the low-, medium- and high-dose QSP groups with QSP at 5, 10 and 20 g/kg/d respectively once a day, and those in the dapoxetine group with dapoxetine hydrochloride at 300 mg/kg at 3 hours before mating. Forty-five female Wistar rats were injected subcutaneously with 20 μg estradiol benzoate after removal of bilateral ovaries to induce estrous estrus. Two and 4 weeks later, the male rats were mated with the female ones for 30 minutes per time and meanwhile observed for the mating behavior of the males, including mounting latency (ML), intromission latency (IL), ejaculation latency (EL), mounting frequency (MF), intromission frequency (IF), and ejaculation frequency (EF). After the 4th week of mating, the hypothalamus of the animals was isolated and weighed, and the content of 5-hydroxytryptamine (5-HT) was measured.
Compared with the blank control group, the PE model controls showed significantly decreased content of 5-HT in the hypothalamus(1 257.1 vs 923.4 ng/g, P<0.05), ML (［11.22 ± 3.60］ vs ［8.69 ± 2.48］ s, P<0.05), IL (［22.33 ± 2.45］ vs ［12.08±1.39］ s, P<0.05), MF (［13.28 ± 3.24］ vs ［7.53 ± 1.84］ times, P<0.05), and EL (［712.35 ± 36.77］ vs ［502.35 ± 46.72］ s, P<0.05). In comparison with the PE model controls, the rats of the QSP and dapoxetine groups exhibited remarkably increased content of 5-HT (P<0.05) and prolonged EL (P<0.05).
Qiaoshao Prescription can prolong EL in PE rats, which might be associated with the increased content of 5-HT in the hypothalamus. Further studies, however, are needed on its underlying mechanisms.
观察巧芍方（QSP）对8-OH-DPAT诱导的雄性大鼠早泄（PE）的干预作用，并探讨其可能的作用机制。
将72只雄性Wistar大鼠随机分为6组，即空白对照组、PE模型对照组、QSP低、中、高剂量组及达泊西汀组。通过向腰骶部脊髓蛛网膜下腔注射8-OH-DPAT建立PE模型。造模4周后，空白对照组和PE模型对照组大鼠以10 ml/kg/d的生理盐水灌胃，QSP低、中、高剂量组大鼠分别以5、10、20 g/kg/d的QSP每日灌胃1次，达泊西汀组大鼠在交配前3小时以300 mg/kg的盐酸达泊西汀灌胃。45只雌性Wistar大鼠双侧卵巢切除后皮下注射20 μg苯甲酸雌二醇诱导发情。2周和4周后，雄性大鼠与雌性大鼠每次交配30分钟，同时观察雄性大鼠的交配行为，包括爬跨潜伏期（ML）、插入潜伏期（IL）、射精潜伏期（EL）、爬跨频率（MF）、插入频率（IF）和射精频率（EF）。交配第4周后，分离动物下丘脑并称重，检测5-羟色胺（5-HT）含量。
与空白对照组相比，PE模型对照组大鼠下丘脑5-HT含量显著降低（1 257.1 vs 923.4 ng/g，P<0.05），ML（［11.22±3.60］vs［8.69±2.48］s，P<0.05）、IL（［22.33±2.45］vs［12.08±1.39］s，P<0.05）、MF（［13.28±3.24］vs［7.53±1.84］次，P<0.05）和EL（［712.35±36.77］vs［502.35±46.72］s，P<0.05）均显著缩短。与PE模型对照组相比，QSP组和达泊西汀组大鼠下丘脑5-HT含量显著升高（P<0.05），EL显著延长（P<0.05）。
巧芍方可延长PE大鼠的EL，这可能与下丘脑5-HT含量增加有关。然而，其潜在机制仍需进一步研究。
