Huynh Florence, Miller David J, Allemann Rudolf K
School of Chemistry, Cardiff University, Cardiff, United Kingdom.
School of Chemistry, Cardiff University, Cardiff, United Kingdom.
Methods Enzymol. 2018;608:83-95. doi: 10.1016/bs.mie.2018.05.004. Epub 2018 Jul 6.
Sesquiterpene synthases catalyze the conversion of farnesyl diphosphate to more than 300 different hydrocarbon and alcohol natural products which often contain multiple fused rings and stereocenters. Recent work has taken advantage of the exquisite stereospecificity of these enzymes to synthesize complex novel sesquiterpenoids using nonnatural substrates. In this chapter, we describe the expression, purification, and use of one such synthase to convert a nonnatural substrate to a novel cyclic ether, thereby expanding the terpenome.
倍半萜合酶催化法呢基二磷酸转化为300多种不同的烃类和醇类天然产物，这些产物通常含有多个稠环和立体中心。最近的研究利用了这些酶的精确立体特异性，使用非天然底物合成复杂的新型倍半萜类化合物。在本章中，我们描述了一种这样的合酶的表达、纯化及其用于将非天然底物转化为新型环醚的过程，从而扩展了萜类化合物组。
