Al Shaye Najla, Migdadi Hussein, Charbaji Asma, Alsayegh Shatha, Daoud Shaza, Al-Anazi Wala, Alghamdi Salem
Department of Biology, Faculty of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, Saudi Arabia.
Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, P.O. Box 11451, Riyadh, Saudi Arabia.
Saudi J Biol Sci. 2018 Sep;25(6):1007-1015. doi: 10.1016/j.sjbs.2018.04.014. Epub 2018 Apr 21.
Genetic diversity among seven Saudi tomato landraces collected from different regions of the country was assessed using SDS-PAGE and molecular (sequence-related amplified polymorphism- SRAP) markers. A total of 19 alternative protein bands with different mobility rates were identified within a molecular weight range of 9.584-225 KDa, with 53% polymorphism. Specific protein bands were observed in the "Hail 548" and "Qatif 565" landraces. Genetic similarity based on Jaccard's coefficient ranged from 0.53 to 1.00, with an average of 0.72. For molecular evaluation, 143 amplicons (fragments) were generated using 27 SRAP primer pair combinations, of which 88 were polymorphic across all the landraces. The PIC values ranged from 0.46 to 0.90, with an average of 0.76. All landraces showed an average of 0.66 similarity coefficient value. The UPGMA dendrogram supported by principal coordinate analysis (PCoA) revealed clusters of the landraces that almost corresponded to their geographical origin. Thus, seed storage protein profiling based on SDS-PAGE and SRAP markers can efficiently be used to assess genetic variability among tomato germplasms. The information obtained in the analysis will be of great interest in the management of collections for utilization in breeding programs or for direct use in quality markets.
利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和分子标记（序列相关扩增多态性-SRAP）评估了从沙特阿拉伯不同地区收集的7个番茄地方品种的遗传多样性。在9.584-225 kDa的分子量范围内共鉴定出19条具有不同迁移率的可变蛋白条带，多态性为53%。在“Hail 548”和“Qatif 565”地方品种中观察到特异性蛋白条带。基于杰卡德系数的遗传相似性范围为0.53至1.00，平均为0.72。为了进行分子评估，使用27对SRAP引物组合产生了143个扩增子（片段），其中88个在所有地方品种中具有多态性。PIC值范围为0.46至0.90，平均为0.76。所有地方品种的相似系数平均值为0.66。由主坐标分析（PCoA）支持的UPGMA聚类图显示，地方品种的聚类几乎与其地理起源相对应。因此，基于SDS-PAGE和SRAP标记的种子贮藏蛋白分析可有效地用于评估番茄种质间的遗传变异性。分析中获得的信息对于管理用于育种计划或直接用于优质市场的种质资源将具有重要意义。
