Amiriyan Marzieh, Shojaeiyan Abdolali, Yadollahi Abbas, Maleki Masoud, Bahari Zeinab
Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Mol Biol Res Commun. 2019 Dec;8(4):181-190.
Fenugreek is one of the important edible and medicinal vegetables that have a long history of cultivation and consumption. Characterize the extent of the genetic diversity among landraces will provide a good context for future breeding programs and genetic resource preservation. Genetic diversity and population structure of 88 individuals of eight landraces of Iranian fenugreek evaluated based on SRAP markers. Seventy-two bands generated from 6 primers in which 56 (80.11%) band were polymorph. Hamadan landrace showed the lowest values of percentage of polymorphic loci (67.86), Nei's gene diversity index (0.24), number of effective alleles (1.40) and Shannon's Information index (0.36). Nei's genetic distance matrix revealed the highest genetic distance between Hamadan and Yazd (0.203) and the highest genetic similarity between Mahallat and Varamin (0.036) landraces. The most gene flow was between Mahallat and Varamin landraces (Nm=8.36) and the least was between Shiraz and Hamadan landraces (Nm=0.66). An extent admixture of alleles between the Iranian fenugreek landraces was observed by the population structure. Mantel test indicated that the genetic differentiation and gene flow is not associated with geographic distance in Iranian fenugreek landraces. Our observations indicated SRAP is an efficient technique to reveal genetic diversity and population structure of Iranian fenugreek landrace.
胡芦巴是重要的食用和药用蔬菜之一，有着悠久的种植和食用历史。表征地方品种间的遗传多样性程度将为未来的育种计划和遗传资源保护提供良好的背景。基于SRAP标记对8个伊朗胡芦巴地方品种的88个个体进行了遗传多样性和群体结构分析。6对引物共产生72条带，其中56条（80.11%）为多态性条带。哈马丹地方品种的多态性位点百分比（67.86）、Nei基因多样性指数（0.24）、有效等位基因数（1.40）和香农信息指数（0.36）均为最低。Nei遗传距离矩阵显示，哈马丹和亚兹德之间的遗传距离最高（0.203），马哈拉特和瓦拉明地方品种之间的遗传相似性最高（0.036）。基因流最大的是马哈拉特和瓦拉明地方品种之间（Nm = 8.36），最小的是设拉子和哈马丹地方品种之间（Nm = 0.66）。通过群体结构观察到伊朗胡芦巴地方品种间存在一定程度的等位基因混合。Mantel检验表明，伊朗胡芦巴地方品种的遗传分化和基因流与地理距离无关。我们的观察表明，SRAP是揭示伊朗胡芦巴地方品种遗传多样性和群体结构的有效技术。
