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来自DNS-seq的染色质结构概况数据:B73玉米(L)四个参考组织的差异核酸酶敏感性图谱

Chromatin structure profile data from DNS-seq: Differential nuclease sensitivity mapping of four reference tissues of B73 maize ( L).

作者信息

Turpin Zachary M, Vera Daniel L, Savadel Savannah D, Lung Pei-Yau, Wear Emily E, Mickelson-Young Leigh, Thompson William F, Hanley-Bowdoin Linda, Dennis Jonathan H, Zhang Jinfeng, Bass Hank W

机构信息

Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA.

Center for Genomics and Personalized Medicine, Florida State University, Tallahassee, FL, USA.

出版信息

Data Brief. 2018 Aug 10;20:358-363. doi: 10.1016/j.dib.2018.08.015. eCollection 2018 Oct.

DOI:10.1016/j.dib.2018.08.015
PMID:30175199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6117953/
Abstract

Presented here are data from Next-Generation Sequencing of differential micrococcal nuclease digestions of formaldehyde-crosslinked chromatin in selected tissues of maize () inbred line B73. Supplemental materials include a wet-bench protocol for making DNS-seq libraries, the DNS-seq data processing pipeline for producing genome browser tracks. This report also includes the peak-calling pipeline using the iSeg algorithm to segment positive and negative peaks from the DNS-seq difference profiles. The data repository for the sequence data is the NCBI SRA, BioProject Accession 8.

摘要

本文展示了来自玉米(Zea mays)自交系B73选定组织中甲醛交联染色质的微球菌核酸酶差异消化的二代测序数据。补充材料包括用于制备DNS-seq文库的湿实验方案、用于生成基因组浏览器轨道的DNS-seq数据处理流程。本报告还包括使用iSeg算法从DNS-seq差异图谱中分割正负峰的峰检测流程。序列数据的存储库是NCBI SRA,生物项目登录号为8。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/554e/6117953/5e08a6ab5cfe/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/554e/6117953/d5ea427d968e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/554e/6117953/5e08a6ab5cfe/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/554e/6117953/d5ea427d968e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/554e/6117953/5e08a6ab5cfe/gr2.jpg

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Proc Natl Acad Sci U S A. 2016 May 31;113(22):E3177-84. doi: 10.1073/pnas.1525244113. Epub 2016 May 16.
3
Differential nuclease sensitivity profiling of chromatin reveals biochemical footprints coupled to gene expression and functional DNA elements in maize.
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Plant Direct. 2021 Aug 16;5(8):e337. doi: 10.1002/pld3.337. eCollection 2021 Aug.
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