Turpin Zachary M, Vera Daniel L, Savadel Savannah D, Lung Pei-Yau, Wear Emily E, Mickelson-Young Leigh, Thompson William F, Hanley-Bowdoin Linda, Dennis Jonathan H, Zhang Jinfeng, Bass Hank W
Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA.
Center for Genomics and Personalized Medicine, Florida State University, Tallahassee, FL, USA.
Data Brief. 2018 Aug 10;20:358-363. doi: 10.1016/j.dib.2018.08.015. eCollection 2018 Oct.
Presented here are data from Next-Generation Sequencing of differential micrococcal nuclease digestions of formaldehyde-crosslinked chromatin in selected tissues of maize () inbred line B73. Supplemental materials include a wet-bench protocol for making DNS-seq libraries, the DNS-seq data processing pipeline for producing genome browser tracks. This report also includes the peak-calling pipeline using the iSeg algorithm to segment positive and negative peaks from the DNS-seq difference profiles. The data repository for the sequence data is the NCBI SRA, BioProject Accession 8.
本文展示了来自玉米(Zea mays)自交系B73选定组织中甲醛交联染色质的微球菌核酸酶差异消化的二代测序数据。补充材料包括用于制备DNS-seq文库的湿实验方案、用于生成基因组浏览器轨道的DNS-seq数据处理流程。本报告还包括使用iSeg算法从DNS-seq差异图谱中分割正负峰的峰检测流程。序列数据的存储库是NCBI SRA,生物项目登录号为8。