Oudemans P B, Brutel de la Rivière G, Hart G A, van Heerde P, Scholte G, Vroom T M
Cancer. 1986 Sep 15;58(6):1252-9. doi: 10.1002/1097-0142(19860915)58:6<1252::aid-cncr2820580613>3.0.co;2-m.
A series of 34 B-cell lymphomas was studied for the presence of transferrin receptors by immunofluorescence on frozen tissue sections with a monoclonal antibody. These lymphomas were classified by light microscopic study and furthermore investigated by immunohistochemical and morphometric methods. There were diffuse centrocytic, diffuse centroblastic, and follicular and diffuse centrocytic/centroblastic lymphomas. Significant differences in log mean nuclear area in these four histologic groups were found, as well as a significant correlation between log nuclear area and transferrin receptors as measured semiquantitatively by fluorescence intensity of the cell membranes. In addition, it was found that there is a strong correlation between the transferrin receptor activity and malignancy grading on histologic and morphometric basis. Therefore, the transferrin receptor determination on frozen sections appears to be a good method of malignancy grading.
运用单克隆抗体，通过对冷冻组织切片进行免疫荧光检测，研究了34例B细胞淋巴瘤中转铁蛋白受体的存在情况。这些淋巴瘤通过光学显微镜研究进行分类，并进一步采用免疫组织化学和形态计量学方法进行研究。其中有弥漫性中心细胞性、弥漫性中心母细胞性、滤泡性以及弥漫性中心细胞性/中心母细胞性淋巴瘤。发现这四个组织学组在对数平均核面积上存在显著差异，并且通过细胞膜荧光强度半定量测量的对数核面积与转铁蛋白受体之间存在显著相关性。此外，还发现转铁蛋白受体活性与基于组织学和形态计量学的恶性分级之间存在很强的相关性。因此，对冷冻切片进行转铁蛋白受体测定似乎是一种很好的恶性分级方法。
