发育中小鼠性腺定量PCR分析中参考基因的鉴定
Identification of reference genes for quantitative PCR analyses in developing mouse gonads.
作者信息
Yokoyama Toshifumi, Omotehara Takuya, Hirano Tetsushi, Kubota Naoto, Yanai Shogo, Hasegawa Chinatsu, Takada Tadashi, Mantani Yohei, Hoshi Nobuhiko
机构信息
Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
Department of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku, Tokyo 160-8402, Japan.
出版信息
J Vet Med Sci. 2018 Nov 1;80(10):1534-1539. doi: 10.1292/jvms.18-0417. Epub 2018 Sep 3.
Abstract
Stable reference genes are important for gene expression analyses such as quantitative PCR. The stability of 15 candidate reference genes that can be used to developing mouse gonads was thoroughly verified using combinations of multiple algorithms. The expression of these genes fluctuated greatly depending on the analysis period and/or gender. Peptidylprolyl isomerase A (Ppia) and polymerase (RNA) II (DNA directed) polypeptide A (Polr2a) were the reference genes that were used stably for a wide analysis period in developing mouse gonads. Furthermore, the stable reference genes corresponding to the analysis period and/or gender were ranked. These results are useful for the selection of the optimal reference gene required for high-precision measurements.
摘要
稳定的参考基因对于诸如定量PCR等基因表达分析非常重要。使用多种算法的组合，对15个可用于发育中小鼠性腺研究的候选参考基因的稳定性进行了全面验证。这些基因的表达根据分析时期和/或性别有很大波动。肽基脯氨酰异构酶A（Ppia）和聚合酶（RNA）II（DNA定向）多肽A（Polr2a）是在发育中小鼠性腺的广泛分析时期内稳定使用的参考基因。此外，还对与分析时期和/或性别相对应的稳定参考基因进行了排名。这些结果对于选择高精度测量所需的最佳参考基因很有用。
相似文献
1
Identification of reference genes for quantitative PCR analyses in developing mouse gonads.发育中小鼠性腺定量PCR分析中参考基因的鉴定
J Vet Med Sci. 2018 Nov 1;80(10):1534-1539. doi: 10.1292/jvms.18-0417. Epub 2018 Sep 3.
2
Determination of reference genes that are independent of feeding rhythms for circadian studies of mouse metabolic tissues.确定用于小鼠代谢组织昼夜节律研究的、与进食节律无关的内参基因。
Mol Genet Metab. 2017 Jun;121(2):190-197. doi: 10.1016/j.ymgme.2017.04.001. Epub 2017 Apr 5.
3
Identification of suitable reference genes in the mouse placenta.小鼠胎盘中合适内参基因的鉴定。
Placenta. 2016 Mar;39:7-15. doi: 10.1016/j.placenta.2015.12.017. Epub 2015 Dec 25.
4
Evaluation of reference genes for quantitative real-time PCR in the brain, pituitary, and gonads of songbirds.鸣禽大脑、垂体和性腺中用于定量实时PCR的内参基因评估
Horm Behav. 2014 Jul;66(2):267-75. doi: 10.1016/j.yhbeh.2014.04.011. Epub 2014 Apr 26.
5
Identification of suitable normalizing genes for quantitative real-time RT-PCR analysis of gene expression in fetal mouse gonads.鉴定用于定量实时 RT-PCR 分析胎儿鼠性腺基因表达的合适内参基因。
Sex Dev. 2009;3(4):194-204. doi: 10.1159/000228720. Epub 2009 Sep 11.
6
Effect of carbon monoxide on gene expression in cerebrocortical astrocytes: Validation of reference genes for quantitative real-time PCR.一氧化碳对大脑皮质星形胶质细胞基因表达的影响：定量实时PCR内参基因的验证
Nitric Oxide. 2015 Sep 15;49:80-9. doi: 10.1016/j.niox.2015.07.003. Epub 2015 Jul 18.
7
The dilution effect and the importance of selecting the right internal control genes for RT-qPCR: a paradigmatic approach in fetal sheep.稀释效应以及为逆转录定量聚合酶链反应选择合适内参基因的重要性：绵羊胎儿中的一种典型方法
BMC Res Notes. 2015 Feb 27;8:58. doi: 10.1186/s13104-015-0973-7.
8
Validating reference genes within a mouse model system of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxicity.验证 2,3,7,8-四氯二苯并对二恶英（TCDD）毒性的小鼠模型系统中的参考基因。
Chem Biol Interact. 2013 Sep 5;205(1):63-71. doi: 10.1016/j.cbi.2013.06.008. Epub 2013 Jun 19.
9
Quantitative evaluation and selection of reference genes in mouse oocytes and embryos cultured in vivo and in vitro.体内和体外培养的小鼠卵母细胞及胚胎中参考基因的定量评估与筛选
BMC Dev Biol. 2007 Mar 6;7:14. doi: 10.1186/1471-213X-7-14.
10
Identification of reference genes for reverse transcription quantitative real-time PCR normalization in pepper (Capsicum annuum L.).辣椒（Capsicum annuum L.）反转录定量实时 PCR 标准化的内参基因的鉴定。
Biochem Biophys Res Commun. 2011 Dec 9;416(1-2):24-30. doi: 10.1016/j.bbrc.2011.10.105. Epub 2011 Nov 6.
引用本文的文献
1
NR2F2 is required in the embryonic testis for fetal Leydig cell development.胚胎睾丸中胎儿睾丸间质细胞的发育需要NR2F2。
Elife. 2025 Jul 10;14:RP103783. doi: 10.7554/eLife.103783.
2
Deferential nephrotoxicity effect of lanthanum oxide nanoparticle responses to concentration and time in vivo.氧化镧纳米颗粒在体内对浓度和时间的差异性肾毒性作用。
Sci Rep. 2025 Jun 6;15(1):19926. doi: 10.1038/s41598-025-04996-0.
3
Ramadan fasting model modulates biomarkers of longevity and metabolism in male obese and non-obese rats.斋月禁食模型调节雄性肥胖和非肥胖大鼠的长寿和代谢生物标志物。
Sci Rep. 2024 Nov 20;14(1):28731. doi: 10.1038/s41598-024-79557-y.
4
Characterization of dUTPase expression in mouse postnatal development and adult neurogenesis.在小鼠出生后发育和成年神经发生过程中 dUTPase 表达的特征。
Sci Rep. 2024 Jun 7;14(1):13139. doi: 10.1038/s41598-024-63405-0.
5
Oral administration of proniosomal glibenclamide formulation protects testicular tissue from hyperglycemia fluctuations and ROS via pathway.口服前体脂质体格列本脲制剂可通过 途径保护睾丸组织免受高血糖波动和活性氧的影响。
Heliyon. 2024 May 17;10(10):e31283. doi: 10.1016/j.heliyon.2024.e31283. eCollection 2024 May 30.
6
Optimal reference genes for RNA tissue analysis in small animal models of hemorrhagic fever viruses.在出血热病毒的小动物模型中进行 RNA 组织分析的最佳参考基因。
Sci Rep. 2023 Nov 8;13(1):19384. doi: 10.1038/s41598-023-45740-w.
7
Selection of species specific panel of reference genes in peripheral blood mononuclear cells of native livestock species adapted to trans-Himalayan region of Leh-Ladakh.选择适应列城-拉达克横断山脉地区的本地牲畜外周血单核细胞中物种特异性参考基因面板。
Sci Rep. 2022 Nov 2;12(1):18473. doi: 10.1038/s41598-022-22588-0.
8
Identification of suitable reference genes for normalization of reverse transcription quantitative real-time PCR (RT-qPCR) in the fibrotic phase of the bleomycin mouse model of pulmonary fibrosis.鉴定博来霉素诱导的小鼠肺纤维化模型纤维化期逆转录实时定量 PCR（RT-qPCR）中合适的内参基因。
PLoS One. 2022 Oct 17;17(10):e0276215. doi: 10.1371/journal.pone.0276215. eCollection 2022.
9
A Short Promoter Region Containing Conserved Regulatory Motifs Is Required for Steroidogenic Acute Regulatory Protein () Gene Expression in the Mouse Testis.一个含有保守调控元件的短启动子区是小鼠睾丸中类固醇急性调节蛋白 (StAR) 基因表达所必需的。
Int J Mol Sci. 2022 Oct 9;23(19):12009. doi: 10.3390/ijms231912009.
10
Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach.采用蛋白质组学方法评价 TILI-2 作为酪氨酸酶抑制剂、抗氧化剂及其在预防黑色素生成中的作用。
Molecules. 2022 May 18;27(10):3228. doi: 10.3390/molecules27103228.
本文引用的文献
1
Ontogenic and morphological study of gonadal formation in genetically-modified sex reversal XY(POS) mice.转基因性反转XY（POS）小鼠性腺形成的个体发生及形态学研究
J Vet Med Sci. 2016 Jan;77(12):1587-98. doi: 10.1292/jvms.15-0292. Epub 2015 Jul 18.
2
Reference gene validation for RT-qPCR, a note on different available software packages.用于逆转录定量聚合酶链反应（RT-qPCR）的内参基因验证，关于不同可用软件包的说明
PLoS One. 2015 Mar 31;10(3):e0122515. doi: 10.1371/journal.pone.0122515. eCollection 2015.
3
Identification and validation of suitable reference genes for RT-qPCR analysis in mouse testis development.鉴定和验证用于小鼠睾丸发育 RT-qPCR 分析的合适参考基因。
Mol Genet Genomics. 2014 Dec;289(6):1157-69. doi: 10.1007/s00438-014-0877-6. Epub 2014 Jun 22.
4
Validation of endogenous normalizing genes for expression analyses in adult human testis and germ cell neoplasms.用于成年人类睾丸和生殖细胞肿瘤表达分析的内源性标准化基因的验证
Mol Hum Reprod. 2014 Aug;20(8):709-18. doi: 10.1093/molehr/gau030. Epub 2014 Apr 17.
5
Five years MIQE guidelines: the case of the Arabian countries.《MIQE指南五年回顾：以阿拉伯国家为例》
PLoS One. 2014 Feb 4;9(2):e88266. doi: 10.1371/journal.pone.0088266. eCollection 2014.
6
Building the mammalian testis: origins, differentiation, and assembly of the component cell populations.构建哺乳动物睾丸：细胞群体的起源、分化和组装。
Genes Dev. 2013 Nov 15;27(22):2409-26. doi: 10.1101/gad.228080.113.
7
Reference genes in real-time PCR.实时 PCR 中的参考基因。
J Appl Genet. 2013 Nov;54(4):391-406. doi: 10.1007/s13353-013-0173-x.
8
Determination of reliable reference genes for multi-generational gene expression analysis on C. elegans exposed to abused drug nicotine.确定用于对暴露于滥用药物尼古丁的秀丽隐杆线虫进行多代基因表达分析的可靠内参基因。
Psychopharmacology (Berl). 2013 Nov;230(1):77-88. doi: 10.1007/s00213-013-3139-0. Epub 2013 May 17.
9
Validation of reference genes for the relative quantification of gene expression in human epicardial adipose tissue.验证人类心外膜脂肪组织中基因表达相对定量的参考基因。
PLoS One. 2012;7(4):e32265. doi: 10.1371/journal.pone.0032265. Epub 2012 Apr 12.
10
Selection of stable reference genes for quantitative rt-PCR comparisons of mouse embryonic and extra-embryonic stem cells.用于定量 RT-PCR 比较小鼠胚胎和胚胎外干细胞的稳定参考基因的选择。
PLoS One. 2011;6(11):e27592. doi: 10.1371/journal.pone.0027592. Epub 2011 Nov 10.
Zotero 插件