Yokoyama Toshifumi, Omotehara Takuya, Hirano Tetsushi, Kubota Naoto, Yanai Shogo, Hasegawa Chinatsu, Takada Tadashi, Mantani Yohei, Hoshi Nobuhiko
Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
Department of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku, Tokyo 160-8402, Japan.
J Vet Med Sci. 2018 Nov 1;80(10):1534-1539. doi: 10.1292/jvms.18-0417. Epub 2018 Sep 3.
Stable reference genes are important for gene expression analyses such as quantitative PCR. The stability of 15 candidate reference genes that can be used to developing mouse gonads was thoroughly verified using combinations of multiple algorithms. The expression of these genes fluctuated greatly depending on the analysis period and/or gender. Peptidylprolyl isomerase A (Ppia) and polymerase (RNA) II (DNA directed) polypeptide A (Polr2a) were the reference genes that were used stably for a wide analysis period in developing mouse gonads. Furthermore, the stable reference genes corresponding to the analysis period and/or gender were ranked. These results are useful for the selection of the optimal reference gene required for high-precision measurements.
稳定的参考基因对于诸如定量PCR等基因表达分析非常重要。使用多种算法的组合，对15个可用于发育中小鼠性腺研究的候选参考基因的稳定性进行了全面验证。这些基因的表达根据分析时期和/或性别有很大波动。肽基脯氨酰异构酶A（Ppia）和聚合酶（RNA）II（DNA定向）多肽A（Polr2a）是在发育中小鼠性腺的广泛分析时期内稳定使用的参考基因。此外，还对与分析时期和/或性别相对应的稳定参考基因进行了排名。这些结果对于选择高精度测量所需的最佳参考基因很有用。
