Roux-Lombard P, Cruchaud A, Dayer J M
Cell Immunol. 1986 Feb;97(2):286-96. doi: 10.1016/0008-8749(86)90399-0.
Both interferon-gamma (IFN-gamma) and 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) induce changes in the human monocytic cell line U937 that may reflect cellular differentiation. The effects of recombinant IFN-gamma and 1 alpha,25(OH)2D3 on U937 cells with regard to the release of superoxide anion (O2-), prostaglandin E2 (PGE2), and mononuclear cell factor (MCF) after stimulation with phorbol myristate acetate (PMA) were examined. PMA did not induce O2- production in untreated cells. A 3-day preincubation with IFN-gamma or 1 alpha,25(OH)2D3 resulted in a 5- to 10-fold increase in PMA-stimulated production of O2- as compared to cells preincubated in medium alone. The response was related to IFN-gamma and 1 alpha,25(OH)2D3 concentrations. In contrast, the PMA-induced production of PGE2 and MCF does not require preincubation with either IFN-gamma or 1 alpha,25(OH)2D3. These results suggest that O2- production and cytokine production (i.e., PGE2 and MCF) are modulated by different signals related to maturation processes.
干扰素-γ（IFN-γ）和1α,25-二羟基维生素D3（1α,25(OH)2D3）均可诱导人单核细胞系U937发生变化，这些变化可能反映细胞分化。研究了重组IFN-γ和1α,25(OH)2D3对U937细胞在用佛波酯肉豆蔻酸酯乙酸酯（PMA）刺激后超氧阴离子（O2-）、前列腺素E2（PGE2）和单核细胞因子（MCF）释放的影响。PMA在未处理的细胞中不诱导O2-产生。与仅在培养基中预孵育的细胞相比，用IFN-γ或1α,25(OH)2D3预孵育3天导致PMA刺激的O2-产生增加5至10倍。该反应与IFN-γ和1α,25(OH)2D3的浓度有关。相反，PMA诱导的PGE2和MCF产生不需要与IFN-γ或1α,25(OH)2D3预孵育。这些结果表明，O2-产生和细胞因子产生（即PGE2和MCF）受与成熟过程相关的不同信号调节。
