γ-干扰素对人单核母细胞系U937细胞分化及细胞因子产生的影响
Effects of interferon-gamma on cell differentiation and cytokine production of a human monoblast cell line, U937.
作者信息
Ishizuka T, Hirata I, Adachi M, Kurimoto F, Hisada T, Dobashi K, Mori M
机构信息
First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
出版信息
Inflammation. 1995 Dec;19(6):627-36. doi: 10.1007/BF01534567.
The U937 cell, a human monoblast cell line, has been used as a model to study the function of human monocytes. We investigated the effects of interferon-gamma (IFN-gamma) on superoxide anion (O2-) production, cell surface antigens, and cytokine production of U937 cells. IFN-gamma treatment enhanced O2- production of fMLP or PMA-stimulated U937 cells. IFN-gamma increased the ratio of CD23 and CD11b positive cells. The fluorescence intensity of CD14 and CD25 was enhanced by IFN-gamma treatment. U937 cells produced IL-1 alpha, IL-1 beta, IL-6, and TNF-alpha by lipopolysaccharide (LPS) stimulation. IFN-gamma treatment enhanced TNF-alpha production, but decreased IL-6 production. These results suggest that IFN-gamma differentiates U937 cells to monocyte-like cells and it regulates the production systems of IL-6 and TNF-alpha separately in U937 cells.
U937细胞是一种人单核母细胞系，已被用作研究人单核细胞功能的模型。我们研究了γ干扰素（IFN-γ）对U937细胞超氧阴离子（O2-）产生、细胞表面抗原和细胞因子产生的影响。IFN-γ处理增强了fMLP或PMA刺激的U937细胞的O2-产生。IFN-γ增加了CD23和CD11b阳性细胞的比例。IFN-γ处理增强了CD14和CD25的荧光强度。U937细胞通过脂多糖（LPS）刺激产生IL-1α、IL-1β、IL-6和TNF-α。IFN-γ处理增强了TNF-α的产生，但降低了IL-6的产生。这些结果表明，IFN-γ将U937细胞分化为单核细胞样细胞，并分别调节U937细胞中IL-6和TNF-α的产生系统。
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