Crustacean Molecular Biology and Genomics Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block 6(th) Floor, Burma Colony, Karaikudi, 630004, Tamil Nadu, India.
Crustacean Molecular Biology and Genomics Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block 6(th) Floor, Burma Colony, Karaikudi, 630004, Tamil Nadu, India.
Fish Shellfish Immunol. 2018 Dec;83:61-75. doi: 10.1016/j.fsi.2018.08.057. Epub 2018 Aug 31.
In the present study, immunoenhancing effect of β-1, 3 glucan binding protein based selenium nanowire (Phβ-GBP-SeNWs) in common carp, Cyprinus carpio was assessed. Biological based selenium nanoform was synthesized, using crustacean immune molecule β-GBP purified from the haemolymph of Paratelphusa hydrodromus. The morphological property of Phβ-GBP-SeNWs was analyzed through TEM which reveals, the synthesized nanowire exhibits approximately 30-50 nm width with smooth surface. For this current study, fish were fed with experimental diet includes Phβ-GBP, sodium selenite, selenomethionine and Phβ-GBP-SeNWs supplemented diet at different concentrations (0.5 mg, 1 mg and 2 mg) for 30 days. The growth performance, cellular and humoral immune responses (myeloperoxidase, reactive oxygen species, alkaline phosphatase and lysozyme activity) and antioxidant enzymes (glutathione peroxidase and catalase activity) in the fish fed with Phβ-GBP-SeNWs supplemented diet were significantly increased in dose-dependent manner, which was observed at two different interval period (15 and 30 day). Also, Phβ-GBP-SeNWs supplemented diet fed fish gain resistant after challenged with aquatic pathogen Aeromonas hydrophila and the relative survival percentage was increased. Agar disc diffusion and BacLight assay clearly demonstrated the antibacterial property of plasma of fish fed with Phβ-GBP-SeNWs supplemented diet against aquatic pathogen A. hydrophila, Vibrio parahaemolyticus and Vibrio alginolyticus. Moreover, confocal laser scanning microscopic analysis clearly showed that, Phβ-GBP-SeNWs supplemented diet fed fish plasma was more efficient in disrupting the architecture of bacterial colonies and thereby reduced the thickness of biofilm. Thus, the present study indicates that, incorporation of Phβ-GBP-SeNWs in the diet enhances the fish immune responses and disease resistance against aquatic pathogens.
本研究评估了β-1,3 葡聚糖结合蛋白基硒纳米线（Phβ-GBP-SeNWs）对鲤鱼的免疫增强作用。采用甲壳动物免疫分子β-GBP 从 Paratelphusa hydrodromus 血淋巴中纯化，合成了基于生物的硒纳米形式。通过 TEM 分析了 Phβ-GBP-SeNWs 的形态特性，结果表明，合成的纳米线具有约 30-50nm 的宽度，表面光滑。在本研究中，鱼被喂食含有 Phβ-GBP、亚硒酸钠、硒代蛋氨酸和 Phβ-GBP-SeNWs 的实验饲料，补充饲料的浓度分别为 0.5mg、1mg 和 2mg，时间为 30 天。用 Phβ-GBP-SeNWs 补充饲料喂养的鱼的生长性能、细胞和体液免疫反应（髓过氧化物酶、活性氧、碱性磷酸酶和溶菌酶活性）和抗氧化酶（谷胱甘肽过氧化物酶和过氧化氢酶活性）显著增加，呈剂量依赖性，在两个不同的间隔期（15 和 30 天）观察到这种情况。此外，用 Phβ-GBP-SeNWs 补充饲料喂养的鱼在受到水生病原体嗜水气单胞菌的挑战后获得了抗性，相对存活率增加。琼脂圆盘扩散和 BacLight 测定清楚地表明，用 Phβ-GBP-SeNWs 补充饲料喂养的鱼的血浆对水生病原体嗜水气单胞菌、副溶血弧菌和溶藻弧菌具有抗菌特性。此外，共聚焦激光扫描显微镜分析清楚地表明，用 Phβ-GBP-SeNWs 补充饲料喂养的鱼的血浆更有效地破坏细菌菌落的结构，从而减少生物膜的厚度。因此，本研究表明，在饲料中加入 Phβ-GBP-SeNWs 可以增强鱼类对水生病原体的免疫反应和抗病能力。
