El-Nobi Gamal, Hassanin Mohammed, Khalil Alshimaa A, Mohammed Alaa Y, Amer Shimaa A, Montaser Metwally M, El-Sharnouby Mohamed E
Department of fish diseases and management, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Antibiotics (Basel). 2021 May 12;10(5):567. doi: 10.3390/antibiotics10050567.
Synbiotic (SYN) additives were assessed as an antibiotic alternative on the effects on the nonspecific immune response and disease resistance of to . Healthy fish (n = 120, average initial weight 18 ± 2 g) were allotted randomly into four experimental groups (3 replicates for each); 1) a control group with no additives (CON), 2) basal diet complemented with 0.1 g kg diets of norfloxacin, NFLX, 3) basal diet fortified with 1 mL kg diet of SYN, and 4) basal diet complemented with a mixture of NFLX and SYN, which was carried out for eight weeks. Results showed a significant increase ( < 0.01) in the serum immune parameters (total protein, globulin and albumin, nitric oxide (NO), and lysozyme activity) in the SYN group and the NFLX+SYN group compared with the CON and NFLX groups. The serum glucose, cholesterol, and triglycerides were higher in NFLX and NFLX+SYN groups than the CON and SYN groups. The catalase (CAT), superoxide dismutase, glutathione peroxidase (GPX) activities were significantly augmented in the NFLX+SYN group, followed by the SYN group compared with CON and NFLX groups. The cumulative mortality rate (CMR) of following the challenge was decreased in the SYN group compared to other groups. The results emphasize that synbiotic could be used as a norfloxacin alternative to enhance the related immunological parameters, including antioxidant activity and disease resistance against infection of .
评估了合生元（SYN）添加剂作为抗生素替代品对[某种鱼]非特异性免疫反应和抗病性的影响。将健康鱼（n = 120，平均初始体重18±2克）随机分为四个实验组（每组3个重复）；1）无添加剂的对照组（CON），2）基础饲料中添加0.1克/千克饲料的诺氟沙星（NFLX），3）基础饲料中添加1毫升/千克饲料的SYN，4）基础饲料中添加NFLX和SYN的混合物，实验持续八周。结果显示，与CON组和NFLX组相比，SYN组和NFLX + SYN组的血清免疫参数（总蛋白、球蛋白和白蛋白、一氧化氮（NO）和溶菌酶活性）显著增加（P < 0.01）。NFLX组和NFLX + SYN组的血清葡萄糖、胆固醇和甘油三酯高于CON组和SYN组。与CON组和NFLX组相比，NFLX + SYN组的过氧化氢酶（CAT）、超氧化物歧化酶、谷胱甘肽过氧化物酶（GPX）活性显著增强，其次是SYN组。与其他组相比，SYN组在[某种细菌]攻毒后的累积死亡率（CMR）降低。结果强调，合生元可作为诺氟沙星的替代品，以增强相关免疫参数，包括抗氧化活性和对[某种鱼]感染[某种细菌]的抗病性。
