College of Food Science and Technology, Central South University of Forestry and Technology, Changsha 410004, PR China; College of Life Sciences and Chemistry, Hunan University of Technology, Hunan 412007, PR China; Hunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan 410004, PR China; State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
College of Food Science and Technology, Central South University of Forestry and Technology, Changsha 410004, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jan 5;206:529-534. doi: 10.1016/j.saa.2018.08.042. Epub 2018 Aug 25.
Hydrogen peroxide (HO), a member of small-molecule reactive oxygen species (ROS), plays an important role in physiological and/or pathological process within live systems. Herein, to quantitatively investigate the biological role of HO in subcellular level, we constructed of a novel two-photon (TP) in near-infrared (NIR) out fluorescent probe (TP-NIR-HO) for visualization of mitochondria HO in living cells and tissues. Specifically, TP-NIR-HO utilized the oxonium ion as the mitochondrial targeting unit and phenylboric acid as the HO reaction moiety. After the phenylboric acid moiety reaction with HO, TP-NIR-HO displayed a ~105-fold fluorescence intensity enhancement in 665 nm. Selectivity experiment demonstrated that the probe can detect HO with high selectivity over other ROS. Moreover, TP-NIR-HO could be employed for imaging HO in mice liver tissues with large tissue-image depth (50-170 μm) under two-photon excitation (800 nm).
过氧化氢（HO）是小分子活性氧（ROS）的成员，在活系统的生理和/或病理过程中发挥重要作用。在此，为了定量研究 HO 在亚细胞水平的生物学作用，我们构建了一种新型的双光子（TP）近红外（NIR）外荧光探针（TP-NIR-HO），用于可视化活细胞和组织中的线粒体 HO。具体而言，TP-NIR-HO 利用氧鎓离子作为线粒体靶向单元，苯硼酸作为 HO 反应部分。苯硼酸部分与 HO 反应后，TP-NIR-HO 在 665nm 处的荧光强度增强了约 105 倍。选择性实验表明，该探针可以高选择性地检测 HO 相对于其他 ROS。此外，TP-NIR-HO 可用于在双光子激发（800nm）下对小鼠肝组织中的 HO 进行成像，具有较大的组织成像深度（50-170μm）。
