State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian 116024, PR China.
School of Life Science and Biotechnology, Dalian University of Technology, 2 Linggong Road, Dalian 116024, PR China.
Biosens Bioelectron. 2017 Aug 15;94:536-543. doi: 10.1016/j.bios.2017.03.039. Epub 2017 Mar 19.
Hydrogen peroxide (HO), one of the reactive oxygen species (ROS), plays vital roles in diverse physiological processes. Imbalance of the HO is concerned with serious diseases such as cardiovascular disorders, neurodegenerative diseases, Alzheimer's disease and cancer. Therefore, it is critical to develop efficient methods for monitoring HO in vivo. In this work, a two-photon excitation (860nm) NIR fluorescent turn-on probe TPNR-HO for HO based on Dicyanomethylene-4H-pyran fluorophore is reported, which can be used in solution detection with 13.2-fold NIR fluorescence enhancement, fast response (completed within 40min), excellent sensitivity (DL 72.48nM), and lower cellular auto-fluorescence interference. Importantly, the perfect photostability of TPNR-HO clearly demonstrated that the probe could be applied to imaging intracellular HO for a long time without photobleaching. In addition, through two-photon imaging, this probe was cell permeable and used to monitor the level of endogenous and exogenous HO with promising biological application.
过氧化氢（HO）是活性氧（ROS）之一，在多种生理过程中发挥着重要作用。HO 的失衡与心血管疾病、神经退行性疾病、阿尔茨海默病和癌症等严重疾病有关。因此，开发有效的方法来监测体内的 HO 至关重要。在这项工作中，报道了一种基于二氰基乙烯-4H-吡喃荧光团的双光子激发（860nm）近红外荧光开启探针 TPNR-HO 可用于溶液检测，具有 13.2 倍的近红外荧光增强、快速响应（在 40min 内完成）、优异的灵敏度（DL 为 72.48nM）和较低的细胞自发荧光干扰。重要的是，TPNR-HO 的完美光稳定性清楚地表明，该探针可以在没有光漂白的情况下长时间应用于细胞内 HO 的成像。此外，通过双光子成像，该探针具有细胞通透性，并可用于监测内源性和外源性 HO 的水平，具有有前景的生物学应用。
