Palomares Roberto A, Gutiérrez-Añez Juan C, Zambrano Sunny, Boscan-Ocando Julio C, Montero Merilio, Camacho Jesús, Perea-Ganchou Fernando P, Ferrer Maria S, Rodríguez-Márquez Jose M, Portillo-Martinez German, Muñoz Rafaela J, Godoy Rosario
Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, USA; Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Unidad de Investigación en Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad del Zulia, Maracaibo, Venezuela.
Anim Reprod Sci. 2018 Oct;197:22-32. doi: 10.1016/j.anireprosci.2018.06.019. Epub 2018 Jun 25.
Criollo Limonero is a tropical Bos taurus breed for sustainable dual purpose (milk and beef) production in the South-American tropics, which is currently threatened with extinction. The objective was to perform a clinical evaluation and histopathological assessment of uterine biopsy samples of repeat breeder (RB) Criollo Limonero cattle to determine the occurrence of pathological conditions as potential causes of subfertility. Twenty-four Criollo Limonero cattle [18 cows (5-13 years old) and 6 heifers (6-7.5 years old)] that had failed to conceive after four or more services were considered for this study. Additionally, five cows with history of adequate reproductive performance were used as a control group. Animals were submitted to physical exam, vaginoscopy, and ultrasonographical evaluation of the reproductive tract. Uterine biopsy samples were collected for histopathological evaluation. Vaginoscopy revealed that 41.7% of the RB cattle had abnormal vaginal secretions, while abnormal secretions were not observed in any control cow. Ultrasonographical examination of the uterus revealed the presence of free uterine fluid in 20.8% of the RB animals, while none of the control cows had fluid in the uterine lumen. In addition, ovarian cysts were observed in 25.0% of the RB animals. Histopathological evaluation of the endometrial biopsies revealed that mononuclear leukocyte infiltration, dilated uterine glands, and periglandular fibrosis were the most prevalent lesions in the sub-fertile animals. Chronic endometritis characterized by inflammatory (mononuclear leukocyte infiltration) and degenerative (dilated glands and periglandular fibrosis) endometrial lesions, and ovarian cysts were the most frequent reproductive pathologies observed in the studied subfertile Criollo Limonero cattle, suggesting a strong association with their reduced fertility.
克里奥洛莱蒙内罗牛是一种热带牛品种，用于南美洲热带地区可持续的双重用途（牛奶和牛肉）生产，目前正面临灭绝的威胁。目的是对反复配种失败（RB）的克里奥洛莱蒙内罗牛的子宫活检样本进行临床评估和组织病理学评估，以确定作为潜在不育原因的病理状况的发生率。本研究纳入了24头克里奥洛莱蒙内罗牛[18头母牛（5 - 13岁）和6头小母牛（6 - 7.5岁）]，这些牛在四次或更多次配种后仍未受孕。此外，选取了五头具有正常繁殖性能历史的母牛作为对照组。对动物进行了体格检查、阴道镜检查和生殖道超声评估。采集子宫活检样本进行组织病理学评估。阴道镜检查显示，41.7%的RB牛有异常阴道分泌物，而对照组母牛未观察到异常分泌物。子宫超声检查发现20.8%的RB动物存在子宫内游离液体，而对照组母牛子宫腔内均无液体。此外，在25.0%的RB动物中观察到卵巢囊肿。子宫内膜活检的组织病理学评估显示，单核白细胞浸润、子宫腺扩张和腺周纤维化是不育动物中最常见的病变。以炎症性（单核白细胞浸润）和退行性（腺扩张和腺周纤维化）子宫内膜病变为特征的慢性子宫内膜炎以及卵巢囊肿是在所研究的不育克里奥洛莱蒙内罗牛中观察到的最常见的生殖病理，表明它们与生育力降低有很强的关联。
