Jaureguiberry M, Giuliodori M J, Mang A V, Madoz L V, Pothmann H, Drillich M, de la Sota R L
Cátedra y Servicio de Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad de La Plata (FCV-UNLP), La Plata, B1900AVW, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, C1033AAJ, Argentina.
Cátedra de Fisiología, FCV-UNLP, La Plata, B1900AVW, Argentina.
J Dairy Sci. 2017 Apr;100(4):3083-3085. doi: 10.3168/jds.2016-11406. Epub 2017 Feb 16.
The objective of the present study was to test the association between the presence of fluid in uterine lumen and the fertility of repeat breeder cows (RBC). Grazing Holstein cows that were clinically normal and were not pregnant after at least 3 consecutive inseminations were defined as RBC and included in the study (n = 358). A cut-point ≥2 mm of uterine lumen visible by ultrasonography was used as indicative of the presence of fluid in the uterus. The effect of this fluid on the fertility of RBC was assessed by mixed logistic regression. Twenty percent of the RBC had ≥2 mm of fluid in their uterus, and this finding was associated with a marked reduction in the odds of conception (odds ratio = 0.46). Additional intrauterine sampling revealed that 24.0% of RBC showed signs of subclinical endometritis [≥5% neutrophils in endometrial cytology (SCE)]. However, we found no agreement between the finding of uterine fluid and the diagnosis of SCE (kappa coefficient = 0.03, 95% confidence interval = -0.07 to 0.14). In conclusion, the presence of fluid (≥2 mm) in uterine lumen, detected by ultrasonography, was associated with lower fertility in RBC, but was not related to the diagnosis of SCE. Thus, ultrasonographic evaluation is an important tool to assist in assessment of RBC.
本研究的目的是检验子宫腔内积液与屡配不孕奶牛（RBC）繁殖力之间的关联。将临床上正常且在至少连续3次人工授精后未怀孕的放牧荷斯坦奶牛定义为RBC并纳入研究（n = 358）。超声检查可见子宫腔≥2 mm的切点被用作子宫内存在液体的指标。通过混合逻辑回归评估这种液体对RBC繁殖力的影响。20%的RBC子宫内有≥2 mm的液体，这一发现与受孕几率显著降低相关（优势比 = 0.46）。额外的子宫内采样显示，24.0%的RBC表现出亚临床子宫内膜炎的迹象[子宫内膜细胞学检查（SCE）中中性粒细胞≥5%]。然而，我们发现子宫积液的发现与SCE的诊断之间没有一致性（kappa系数 = 0.03，95%置信区间 = -0.07至0.14）。总之，超声检查发现子宫腔内存在液体（≥2 mm）与RBC的繁殖力较低有关，但与SCE的诊断无关。因此，超声评估是协助评估RBC的重要工具。
