Pothmann H, Prunner I, Wagener K, Jaureguiberry M, de la Sota R L, Erber R, Aurich C, Ehling-Schulz M, Drillich M
Clinical Unit for Herd Health Management in Ruminants, University Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria.
Clinical Unit for Herd Health Management in Ruminants, University Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria; Functional Microbiology, Institute of Microbiology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria.
Theriogenology. 2015 May;83(8):1249-53. doi: 10.1016/j.theriogenology.2015.01.013. Epub 2015 Jan 16.
The objectives of this study were to assess the prevalence of subclinical endometritis and the presence of common uterine pathogens in repeat breeder cows. A total of 121 cows with three or more consecutive artificial inseminations without conception and no clinical signs of disease were defined as repeat breeder cows and were enrolled in this trial. Intrauterine samples were collected with the cytobrush technique to determine the prevalence of subclinical endometritis and bacteriologic infections. Blood samples were analyzed for concentrations of progesterone and estradiol in plasma to assess ovarian activity. Furthermore, breed, parity, history of calving and postpartum uterine infection, clinical findings of transrectal palpation, and backfat thickness were analyzed as potential factors for the prevalence of subclinical endometritis in repeat breeder cows. The prevalence of subclinical endometritis in repeat breeder cows was 12.7%; but common uterine pathogens, Escherichia coli and Trueperella pyogenes, were found in only one and three cows, respectively. Ovarian activity was determined in 95.0% of all cows. Recorded variables had no effect on the prevalence of subclinical endometritis in repeat breeder cows. In conclusion, subclinical endometritis and uterine infections linked to common pathogens were playing a minor role as a cause for repeat breeder cows in this study. Alternative reasons for failure to conceive in these cows are discussed.
本研究的目的是评估经产不孕奶牛亚临床子宫内膜炎的患病率以及常见子宫病原体的存在情况。共有121头奶牛被定义为经产不孕奶牛，这些奶牛连续进行了三次或更多次人工授精但未受孕且无疾病临床症状，并被纳入本试验。采用细胞刷技术采集子宫内样本，以确定亚临床子宫内膜炎和细菌感染的患病率。分析血液样本中血浆孕酮和雌二醇的浓度，以评估卵巢活性。此外，还分析了品种、胎次、产犊史和产后子宫感染、直肠触诊的临床结果以及背膘厚度等因素，作为经产不孕奶牛亚临床子宫内膜炎患病率的潜在影响因素。经产不孕奶牛中亚临床子宫内膜炎的患病率为12.7%；但常见的子宫病原体，大肠杆菌和化脓隐秘杆菌，分别仅在1头和3头奶牛中被发现。所有奶牛中有95.0%的卵巢活性得到了测定。记录的变量对经产不孕奶牛中亚临床子宫内膜炎的患病率没有影响。总之，在本研究中，亚临床子宫内膜炎和与常见病原体相关的子宫感染作为经产不孕奶牛不孕原因的作用较小。文中讨论了这些奶牛受孕失败的其他原因。
