Institut de Biologia, Universitat de Barcelona, Spain.
Département d'Ecologie Sensorielle, UMR 1392, Institut d'Ecologie et des Sciences de l'Environnement de Paris, Université Paris VI, France.
FEBS J. 2018 Nov;285(21):4019-4040. doi: 10.1111/febs.14648. Epub 2018 Sep 28.
In the male moth, Agrotis ipsilon, the behavioural response and neuron sensitivity within the olfactory centres, the antennal lobes (ALs), to female sex pheromone increase with age, in correlation with the maturation of sex accessory glands (SAGs). By contrast, newly mated males cease to be attracted to sex pheromone and remate when their SAGs are refilled during the next night. The insect hormone receptor 38 (HR38), an ortholog of the vertebrate NR4A receptors, is a component of ecdysteroid signalling pathway which controls adult male physiology and behaviour. Here, we cloned the A. ipsilon HR38 (AiHR38) and explored its function in the coordination of reproductive events in the male. AiHR38 was detected in SAGs and ALs, and where its amount raised with age, in parallel with SAG protein content and sex pheromone responsiveness. By contrast, the AL and SAG AiHR38 expressions declined at 0-2 h after mating, in linking with depletion of SAG protein reserves and loss of sensitivity to sex pheromone. The increased AL and SAG AiHR38 expressions at 20-24 h postmating coincided with replenishing of SAGs and recovery of sensitivity to sex pheromone for a new mating. Moreover, AiHR38 knockdown resulted in reduction in SAG protein amount and disruption of sex pheromone-orientated flight. These results show that the insect HR38 is essential both for SAG activity, probably by controlling the protein synthesis, and display of male sexual behaviour, and that the concomitant regulation of its expression within SAGs and olfactory centres contributes to synchronisation between fertility and sexual activity. DATABASE: The nucleotide sequence of Agrotis ipsilon HR38 is available in the DDBJ/EMBL/GenBank databases under the accession number MF402845.
在雄性斜纹夜蛾（Agrotis ipsilon）中，触角叶（ALs）中的嗅觉中枢对雌性信息素的行为反应和神经元敏感性会随着年龄的增长而增加，这与性附腺（SAGs）的成熟有关。相比之下，刚交配过的雄性在第二天晚上 SAGs 被重新填满时，就不再被性信息素吸引并重新交配。昆虫激素受体 38（HR38）是脊椎动物 NR4A 受体的同源物，是控制成年雄性生理和行为的蜕皮激素信号通路的一个组成部分。在这里，我们克隆了斜纹夜蛾 HR38（AiHR38），并探索了它在协调雄性生殖事件中的功能。AiHR38 在 SAGs 和 ALs 中被检测到，其数量随着年龄的增长而增加，与 SAG 蛋白含量和性信息素反应性平行。相比之下，交配后 0-2 小时，AL 和 SAG 的 AiHR38 表达下降，与 SAG 蛋白储备的消耗和对性信息素敏感性的丧失有关。交配后 20-24 小时，AL 和 SAG 的 AiHR38 表达增加，与 SAG 的补充和对性信息素的敏感性恢复以进行新的交配有关。此外，AiHR38 的敲低导致 SAG 蛋白含量减少和性信息素定向飞行中断。这些结果表明，昆虫 HR38 对于 SAG 活性和雄性性行为的表现都是必不可少的，它的表达在 SAGs 和嗅觉中枢中的同时调节有助于生育力和性活动之间的同步。数据库：斜纹夜蛾 HR38 的核苷酸序列已在 DDBJ/EMBL/GenBank 数据库中作为 MF402845 号登录号可用。
