Bozzolan F, Durand N, Demondion E, Bourgeois T, Gassias E, Debernard S
UMR 1392, Institut d'Ecologie et des Sciences de l'Environnement de Paris, Département d'Ecologie Sensorielle, Université Paris VI, Paris, France.
Laboratoire de Biologie des Ligneux et des Grandes Cultures, INRA, Université d'Orléans, Orléans, France.
Insect Mol Biol. 2017 Aug;26(4):403-413. doi: 10.1111/imb.12303. Epub 2017 Mar 29.
The oestrogen receptor-related receptors (ERRs) are orphan nuclear receptors that were originally identified on the basis of their close homology to the oestrogen receptors. The three mammalian ERR genes participate in the regulation of vital physiological processes including reproduction, development and metabolic homeostasis. Although unique ERRs have been found in insects, data on the function and regulation of these receptors remain sparse. In the present study, a 2095-bp full-length cDNA encoding an ERR, termed AiERR, was isolated from males of the moth Agrotis ipsilon and deposited in the GenBank database under the accession number KT944662. The predicted AiERR protein shared an overall identity of 47-82% with other known insect and mammalian ERR homologues. AiERR exhibited a broad tissue expression pattern with the detection of one transcript of approximately 2 kb in the primary olfactory centres, the antennal lobes (AL). In adult males, the amount of AiERR mRNA in the AL increased concomitantly with age and responses to the female-emitted sex pheromone. Moreover, AiERR knockdown induced an inhibition in the sex pheromone-orientated flight of male. Using A. ipsilon as a model, our study demonstrates that the insect ERR is critical for the performance of male sexual behaviour, probably by acting on central pheromone processing.
雌激素受体相关受体（ERRs）是孤儿核受体，最初是根据它们与雌激素受体的高度同源性而被鉴定出来的。三种哺乳动物ERR基因参与调控包括生殖、发育和代谢稳态在内的重要生理过程。尽管在昆虫中发现了独特的ERRs，但关于这些受体的功能和调控的数据仍然稀少。在本研究中，从黑地老虎雄蛾中分离出一个编码ERR的2095 bp全长cDNA，命名为AiERR，并以登录号KT944662保存在GenBank数据库中。预测的AiERR蛋白与其他已知昆虫和哺乳动物ERR同源物的总体一致性为47%-82%。AiERR表现出广泛的组织表达模式，在主要嗅觉中枢触角叶（AL）中检测到一个约2 kb的转录本。在成年雄蛾中，AL中AiERR mRNA的量随年龄增长以及对雌蛾释放的性信息素的反应而增加。此外，敲低AiERR会导致雄蛾性信息素导向飞行受到抑制。以黑地老虎为模型，我们的研究表明，昆虫ERR可能通过作用于中枢信息素处理过程，对雄性性行为的表现至关重要。
