斯洛伐克首例免疫抑制和免疫功能正常人群中微小隐孢子虫动物基因型的报告。
The first report of animal genotypes of Cryptosporidium parvum in immunosuppressed and immunocompetent humans in Slovakia.
机构信息
The University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia.
University of Pavol Jozef Šafárik, Košice, Slovakia.
出版信息
Transbound Emerg Dis. 2019 Jan;66(1):243-249. doi: 10.1111/tbed.13009. Epub 2018 Sep 17.
The aim of our study was to determine species and genotypes of Cryptosporidium in patients suffering from immunosuppressive illnesses, but also in immunocompetent patients suffering from diarrhoea. A total of 80 samples of faeces were collected from both immunosuppressed and immunocompetent patients. The immunosuppressed patients (65 samples) - 35 adult patients (group A) and 30 children (group B) were hospitalized at the Clinic of Oncohemathology. Samples from immunocompetent humans (15 samples, group C) were taken from patients with clinical signs of acute diarrhoea. With the use of molecular methods targeting the 60 kDa glycoprotein (GP60) gene region, we have identified multiple genotypes of Cryptosporidium. parvum and Cryptosporidium. hominis in immunocompromised, but also in immunocompetent individuals (C. hominis IbA10G2, IeA12G3T3; C. parvum IIaA10G1R1, IIaA11G2R1, IIaA12G2R1, IIaA13G1R1, IIaA14G1R1, IIaA14G2R1, IIaA17G1R1 and IIaA18G1R1). This is the first report of the occurrence of genotypes IIaA10G1R1, IIa12G2R1 and IIaA18G1R1 in human hosts.
我们的研究目的是确定患有免疫抑制性疾病的患者以及免疫功能正常的腹泻患者中隐孢子虫的种属和基因型。共采集了 80 份粪便样本，分别来自免疫抑制和免疫功能正常的患者。免疫抑制患者（65 份样本） - 35 名成年患者（A 组）和 30 名儿童（B 组）在血液肿瘤科住院。免疫功能正常的人类样本（15 份，C 组）来自有急性腹泻临床症状的患者。通过针对 60kDa 糖蛋白（GP60）基因区域的分子方法，我们鉴定出了多种隐孢子虫基因型。在免疫功能低下和免疫功能正常的个体中都发现了隐孢子虫。和隐孢子虫。人（C. hominis IbA10G2、IeA12G3T3；C. parvum IIaA10G1R1、IIaA11G2R1、IIaA12G2R1、IIaA13G1R1、IIaA14G1R1、IIaA14G2R1、IIaA17G1R1 和 IIaA18G1R1）。这是首次在人类宿主中发现基因型 IIaA10G1R1、IIa12G2R1 和 IIaA18G1R1。
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