Department of Epidemiology, Faculty of Medicine, Pavol Jozef Safarik University in Kosice, Trieda SNP1, 040 11, Kosice, Slovakia.
Clinic of Haematology and Oncohaematology, Faculty of Medicine, Pavol Jozef Safarik University and Louis Pasteur University Hospital in Kosice, Trieda SNP 1, 040 11, Kosice, Slovakia.
Parasitol Res. 2022 Jan;121(1):471-476. doi: 10.1007/s00436-021-07294-6. Epub 2021 Nov 4.
This study aimed to determine the presence of cryptosporidiosis in immunosuppressed patients hospitalized at the Clinic of Haematology and Oncohaematology in a form of routine screening. Samples were collected from November 2019 to February 2020, when the first wave of the Coronavirus pandemic occurred in Slovakia. A total of 36 samples were collected from patients hospitalized at the Clinic of Haematology and Oncohaematology, both from the open ward and the intensive care unit. For the diagnosis of cryptosporidiosis, a nested PCR targeting the gp60 gene and the SSU rRNA locus was used. From the 36 samples, Cryptosporidium parvum subtype IIaA17G1R1 was diagnosed in 9 patients (7 from the open ward and 2 from the intensive care unit), all hospitalized at the clinic at the same time, in February 2020. The occurrence of the same species and subtype, Cryptosporidium parvum IIaA17G1R1, in 9 patients hospitalized at the same time, both at the open ward and the intensive care unit may suggest a possible transmission occurred at the clinic.
本研究旨在通过常规筛查的方式，确定在斯洛伐克冠状病毒大流行第一波期间（2019 年 11 月至 2020 年 2 月）于血液学和肿瘤血液学诊所住院的免疫抑制患者是否存在隐孢子虫病。共采集了 36 名来自血液学和肿瘤血液学诊所的住院患者样本，包括普通病房和重症监护病房的患者。为了诊断隐孢子虫病，我们使用了针对 gp60 基因和 SSU rRNA 基因座的巢式 PCR 方法。从这 36 个样本中，我们在 9 名患者（7 名来自普通病房，2 名来自重症监护病房）中诊断出了微小隐孢子虫 IIaA17G1R1 亚型，这些患者均于 2020 年 2 月在诊所住院。9 名同时在普通病房和重症监护病房住院的患者感染了相同的物种和亚型（微小隐孢子虫 IIaA17G1R1），这可能提示在诊所发生了潜在的传播。
