Centre of Research in Neuroscience Lyon, UMR CNRS 5292 - INSERM U 1028, Université Claude Bernard Lyon 1, 50 avenue Tony Garnier , 69366 Lyon , France.
Laboratoire de Neurosciences Cognitives et Adaptatives, UMR 7364, Université de Strasbourg - CNRS, Faculté de Psychologie, 12 rue Goethe 67000 Strasbourg , France.
ACS Chem Neurosci. 2019 Apr 17;10(4):1929-1934. doi: 10.1021/acschemneuro.8b00314. Epub 2018 Sep 10.
Previous work has shown that β-adrenergic and GABAergic systems in the basolateral amygdala (BLA) are involved in the acquisition of conditioned odor aversion (COA) learning. The involvement of α-adrenoreceptors, however, is poorly documented. In a first experiment, male Long-Evans rats received infusions of 0.1 μg of the selective α-antagonist dexefaroxan (Dex) in the BLA before being exposed to COA learning. In a second experiment, levels of norepinephrine (NE) were analyzed following Dex retrodialysis into the BLA. While microdialysis data showed a significant enhancement of NE release in the BLA with Dex, behavioral results showed that pre-CS infusion of Dex impaired, rather than facilitated, the acquisition of COA. Our results show that the NE system in the BLA is involved in the acquisition of COA, including a strong α-receptor modulation until now unsuspected. Supported by the recent literature, the present data suggest moreover that the processes underlying this learning are probably mediated by the balanced effects of NE excitatory/inhibitory signaling in the BLA, in which interneurons are highly involved.
先前的工作表明，外侧杏仁核（BLA）中的β-肾上腺素能和 GABA 能系统参与了条件性厌恶学习（COA）的获得。然而，α-肾上腺素受体的参与情况记录甚少。在第一个实验中，雄性 Long-Evans 大鼠在接受 COA 学习之前，BLA 中接受了 0.1μg 的选择性α拮抗剂 dexefaroxan（Dex）的输注。在第二个实验中，在 Dex 逆行透析到 BLA 后分析去甲肾上腺素（NE）的水平。虽然微透析数据显示 Dex 使 BLA 中的 NE 释放显著增强，但行为结果表明，Dex 预刺激 CS 会损害而不是促进 COA 的获得。我们的结果表明，BLA 中的 NE 系统参与了 COA 的获得，包括迄今为止尚未被怀疑的强烈的α受体调制。此外，最近的文献支持这一观点，即这种学习所涉及的过程可能是由 BLA 中 NE 兴奋/抑制信号的平衡作用介导的，其中中间神经元高度参与其中。
