Tobey K L, Grant G A
J Biol Chem. 1986 Sep 15;261(26):12179-83.
The nucleotide sequence of serA, the structural gene of Escherichia coli which codes for D-3-phosphoglycerate dehydrogenase, has been determined. The structural gene contains 1233 nucleotides which code for the 409 amino acids of the subunit of the tetrameric enzyme, as well as the initiator methionine, which is cleaved from the mature protein, and the termination codon. The majority of the primary structure of the enzyme has been confirmed by automated Edman degradation of peptide fragments produced by a variety of cleavage agents. Comparison of the amino acid sequence of phosphoglycerate dehydrogenase with other NAD-dependent oxidoreductases reveals less than 20% homology, although conservation of certain specific residues in the coenzyme binding domain appears to be evident.
已确定大肠杆菌中编码D-3-磷酸甘油酸脱氢酶的结构基因serA的核苷酸序列。该结构基因包含1233个核苷酸，编码四聚体酶亚基的409个氨基酸，以及从成熟蛋白中切割下来的起始甲硫氨酸和终止密码子。通过对多种裂解剂产生的肽片段进行自动Edman降解，已证实了该酶大部分的一级结构。磷酸甘油酸脱氢酶的氨基酸序列与其他NAD依赖性氧化还原酶的比较显示，同源性不到20%，尽管辅酶结合域中某些特定残基的保守性似乎很明显。
