Rounds M A, Kopaciewicz W, Regnier F E
J Chromatogr. 1986 Jul 18;362(2):187-96. doi: 10.1016/s0021-9673(01)86967-3.
Properties of the matrix and stationary phase which affect the intrinsic loading capacity of silica-based packing materials for preparative anion-exchange chromatography of proteins were investigated. Polyethyleneimine-coated controlled porosity glass beads ranging from 100 to 2000 A in pore diameter were used to evaluate the effects of pore diameter and surface area. Protein binding was found to depend on accessible, rather than total, support surface area. Consequently, wide-pore, high surface area media provide maximum intrinsic loading capacity. Increasing the number of positively charged sites on the stationary phase by increased coating or by quaternization of amines increases hemoglobin-binding capacity.
研究了基质和固定相的性质对基于硅胶的填充材料用于蛋白质制备型阴离子交换色谱的固有负载能力的影响。使用孔径范围为100至2000埃的聚乙烯亚胺涂层的可控孔隙率玻璃珠来评估孔径和表面积的影响。发现蛋白质结合取决于可及的载体表面积，而非总表面积。因此，宽孔、高表面积的介质具有最大的固有负载能力。通过增加涂层或使胺季铵化来增加固定相上带正电位点的数量，可提高血红蛋白结合能力。
