Tosato G, Marti G E, Yarchoan R, Heilman C A, Wang F, Pike S E, Korsmeyer S J, Siminovitch K
J Immunol. 1986 Sep 15;137(6):2037-42.
Most continuous cell lines derived by EBV immortalization of peripheral blood cells are composed of phenotypically mature B lymphocytes, and secrete Ig. Occasionally, EBV-immortalized cell lines have failed to secrete Ig. Expansion and characterization of one of these EBV-induced cell lines, VDS-O, showed that in addition to a lack of Ig secretion, surface and intracytoplasmic Ig were absent. Cell surface phenotyping revealed that VDS-O belongs to the B cell lineage, because it expresses the B cell restricted antigens B1 and B4, while it lacks T cell and monocyte-associated determinants. Analysis of the Ig gene organization in VDS-O revealed that the Ig genes are rearranged for both heavy (gamma) and light (kappa) chains. However, the expected gamma-heavy chain and/or kappa-light chain RNA species were not detected. These findings demonstrate the existence in normal peripheral blood of cells of B cell lineage susceptible to EBV immortalization that have Ig genes that are rearranged but are nonproductive.
大多数通过EB病毒使外周血细胞永生化而获得的连续细胞系由表型成熟的B淋巴细胞组成，并分泌免疫球蛋白（Ig）。偶尔，EB病毒永生化的细胞系无法分泌Ig。对其中一个EB病毒诱导的细胞系VDS - O进行扩增和特性分析表明，除了缺乏Ig分泌外，表面和胞质内Ig也不存在。细胞表面表型分析显示VDS - O属于B细胞谱系，因为它表达B细胞限制性抗原B1和B4，而缺乏T细胞和单核细胞相关的决定簇。对VDS - O中Ig基因组织的分析表明，Ig基因的重链（γ）和轻链（κ）均发生了重排。然而，未检测到预期的γ - 重链和/或κ - 轻链RNA种类。这些发现证明在正常外周血中存在易受EB病毒永生化影响的B细胞谱系细胞，其Ig基因发生了重排但无功能。
