Rana Kalpana, Thaper Deepali, Vander Harpreet, Prabha Vijay
Department of Microbiology, Panjab University, Chandigarh, 160014, India.
Department of Microbiology, Panjab University, Chandigarh, 160014, India.
Reprod Biol. 2018 Dec;18(4):450-455. doi: 10.1016/j.repbio.2018.08.002. Epub 2018 Sep 1.
The present study was designed to evaluate the effect of P. aeruginosa on reproductive potential of male mice via a series of in vitro and in vivo experiments. In vitro studies involved sperm parameters, MgATPase activity and acrosome status. In vivo study employed male mice which in the right vas deferens received 20 μl of either PBS (Group I) or 10 cfu of P. aeruginosa (Group II). The animals were sacrificed on day 3, 7 and 14 and various parameters viz. body weight, TSI (%), bacterial load, spermiogram {i.e. sperm count, motility (%), viability (%) and morphology}, lipid peroxidation and tissue histopathology were evaluated. The results revealed that cell free supernatant of P. aeruginosa resulted in reduced motility, viability, Mgdependent ATPase activity and premature acrosomal loss of mouse spermatozoa in vitro. In vivo study showed that in comparison to group I, group II revealed significant alterations in all the parameters on all the days of sacrifice. Further, when reproductive organs of right and left side of mice in group II were compared, the right side demonstrated more devastating effects in terms of altered TSI (%) of testis and cauda epididymis, higher bacterial counts, azoospermia, increased malondialdehyde levels and severe inflammation in tissue histopathology in comparison to left side where bacteria disseminated in reduced numbers, thereby, resulting in insignificant changes in TSI (%), spermiogram, malondialdehyde levels and tissue histology. This study demonstrates that the colonization of P. aeruginosa in male genital tract could be a risk factor for fertility.
本研究旨在通过一系列体外和体内实验，评估铜绿假单胞菌对雄性小鼠生殖潜能的影响。体外研究涉及精子参数、MgATP酶活性和顶体状态。体内研究使用雄性小鼠，右侧输精管分别注射20μl PBS（第一组）或10 cfu铜绿假单胞菌（第二组）。在第3、7和14天处死动物，并评估各种参数，即体重、睾丸支持细胞指数（TSI，%）、细菌载量、精子图（即精子计数、活力（%）、存活率（%）和形态）、脂质过氧化和组织病理学。结果显示，铜绿假单胞菌的无细胞上清液在体外导致小鼠精子活力降低、存活率降低、镁依赖性ATP酶活性降低和顶体过早丢失。体内研究表明，与第一组相比，第二组在所有处死日的所有参数上均有显著变化。此外，比较第二组小鼠左右两侧的生殖器官时，右侧睾丸和附睾尾部的TSI（%）变化、细菌计数更高、无精子症、丙二醛水平升高以及组织病理学中的严重炎症方面表现出更具破坏性的影响，而左侧细菌扩散数量减少，因此TSI（%）、精子图、丙二醛水平和组织学变化不显著。本研究表明，铜绿假单胞菌在男性生殖道中的定植可能是生育的一个危险因素。
