Martinac Boris, Bavi Navid, Ridone Pietro, Nikolaev Yury A, Martinac Adam D, Nakayama Yoshitaka, Rohde Paul R, Bavi Omid
Victor Chang Cardiac Research Institute, Lowy Packer Building, Darlinghurst, NSW, 2010, Australia.
St Vincent's Clinical School, University of New South Wales, 405 Liverpool St, Darlinghurst, NSW, 2010, Australia.
Biophys Rev. 2018 Oct;10(5):1377-1384. doi: 10.1007/s12551-018-0450-3. Epub 2018 Sep 4.
Mechanical stimuli acting on the cellular membrane are linked to intracellular signaling events and downstream effectors via different mechanoreceptors. Mechanosensitive (MS) ion channels are the fastest known primary mechano-electrical transducers, which convert mechanical stimuli into meaningful intracellular signals on a submillisecond time scale. Much of our understanding of the biophysical principles that underlie and regulate conversion of mechanical force into conformational changes in MS channels comes from studies based on MS channel reconstitution into lipid bilayers. The bilayer reconstitution methods have enabled researchers to investigate the structure-function relationship in MS channels and probe their specific interactions with their membrane lipid environment. This brief review focuses on close interactions between MS channels and the lipid bilayer and emphasizes the central role that the transbilayer pressure profile plays in mechanosensitivity and gating of these fascinating membrane proteins.
作用于细胞膜的机械刺激通过不同的机械感受器与细胞内信号事件和下游效应器相连。机械敏感（MS）离子通道是已知最快的初级机械电换能器，它能在亚毫秒时间尺度上将机械刺激转化为有意义的细胞内信号。我们对机械力转化为MS通道构象变化背后及调节该过程的生物物理原理的许多理解，都来自于将MS通道重组到脂质双层中的研究。双层重组方法使研究人员能够研究MS通道中的结构 - 功能关系，并探究它们与膜脂质环境的特定相互作用。本简要综述聚焦于MS通道与脂质双层之间的紧密相互作用，并强调跨双层压力分布在这些迷人膜蛋白的机械敏感性和门控中所起的核心作用。
