Jiang Yirong, Mao Shuqin, Huang Weisu, Lu Baiyi, Cai Zengxuan, Zhou Fei, Li Maiquan, Lou Tiantian, Zhao Yajing
College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation, Zhejiang University , Hangzhou 310058, China.
Department of Applied Technology, Zhejiang Economic & Trade Polytechnic , Hangzhou 310018, China.
J Agric Food Chem. 2016 Mar 30;64(12):2459-66. doi: 10.1021/acs.jafc.5b03474. Epub 2015 Sep 8.
Variations of phenylethanoid glycoside profiles and antioxidant activities in Osmanthus fragrans flowers through the digestive tract were evaluated by a simulated digestion model and UPLC/PDA/MS. Major phenylethanoid glycosides and phenolic acids, namely, salidroside, acteoside, isoacteoside, chlorogenic acid, and caffeic acid, were identified in four cultivars of O. fragrans flowers, and the concentration of acteoside was the highest, being up to 71.79 mg/g dry weight. After simulated digestion, total phenylethanoid glycoside contents and antioxidant activities were significantly decreased. Acteoside was identified as decomposing into caffeic acid, whereas salidroside was found to be stable during simulated digestion. According to Pearson's correlation analysis, acteoside contents showed good correlations with antioxidant activities during simulated digestion (R(2) = 0.994, P < 0.01). In conclusion, acteoside was the major contributor to the antioxidant activity of O. fragrans flowers, and salidroside was considered as the major antioxidant compound of O. fragrans flowers in vivo.
通过模拟消化模型和超高效液相色谱/光电二极管阵列/质谱联用技术（UPLC/PDA/MS），评估了桂花在消化道中苯乙醇苷谱和抗氧化活性的变化。在四个桂花品种的花朵中鉴定出了主要的苯乙醇苷和酚酸，即红景天苷、毛蕊花糖苷、异毛蕊花糖苷、绿原酸和咖啡酸，其中毛蕊花糖苷的含量最高，达71.79毫克/克干重。模拟消化后，苯乙醇苷的总含量和抗氧化活性显著降低。毛蕊花糖苷被鉴定分解为咖啡酸，而红景天苷在模拟消化过程中被发现是稳定的。根据皮尔逊相关性分析，模拟消化过程中毛蕊花糖苷含量与抗氧化活性呈现良好的相关性（R² = 0.994，P < 0.01）。总之，毛蕊花糖苷是桂花抗氧化活性的主要贡献者，红景天苷被认为是桂花在体内的主要抗氧化化合物。
