Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong.
Lab Chip. 2018 Oct 9;18(20):3074-3078. doi: 10.1039/c8lc00726h.
We developed a highly efficient method for patterning cells by a novel and simple technique called lift-off cell lithography (LCL). Our approach borrows the key concept of lift-off lithography from microfabrication and utilizes a fully biocompatible process to achieve high-throughput, high-efficiency cell patterning with nearly zero background defects across a large surface area. Using LCL, we reproducibly achieved >70% patterning efficiency for both adherent and non-adherent cells with <1% defects in undesired areas.
我们开发了一种高效的细胞图案化方法，该方法采用一种新颖而简单的技术，称为离型细胞光刻（LCL）。我们的方法借鉴了微制造中的离型光刻的关键概念，并利用完全兼容生物的工艺，在大面积上实现高通量、高效率的细胞图案化，几乎没有背景缺陷。使用 LCL，我们可重复地实现了对贴壁和非贴壁细胞的＞70%的图案化效率，在不需要的区域的缺陷＜1%。
