Giesen-Crouse E M, Fandeleur P, Imbs J L
J Pharmacol. 1986 Apr-Jun;17(2):146-8.
We had previously demonstrated that (3H)-piretanide binds to a receptor located on medullary membranes of canine kidney. Here we show that binding is specific for a particular group of loop diuretics. Sulfonamide diuretics of the benzoic acid family, other substituted sulfonamides, and phenoxy-acetic acid derivates displace (3H)-piretanide from its receptor. Loop diuretics that do not act at the luminal tubular membrane do not displace piretanide, nor do diuretics with a different site and mode of action (thiazides; inhibitors of the Na+/H+ antiporter (amiloride), of Na+K+ ATPase (ouabain), or of carbonic anhydrase (acetazolamide). We demonstrate that no interference occurs between the piretanide receptor and membrane bound receptors of several neurotransmitters.
我们之前已经证明，(3H)-吡咯他尼与位于犬肾髓质膜上的一种受体结合。在此我们表明，这种结合对特定的一类袢利尿剂具有特异性。苯甲酸类的磺胺利尿剂、其他取代磺胺以及苯氧乙酸衍生物可将(3H)-吡咯他尼从其受体上置换下来。作用于肾小管管腔膜的袢利尿剂不能置换吡咯他尼，作用位点和作用方式不同的利尿剂（噻嗪类；Na+/H+ 反向转运体抑制剂（阿米洛利）、Na+K+ ATP酶抑制剂（哇巴因）或碳酸酐酶抑制剂（乙酰唑胺））也不能置换吡咯他尼。我们证明，吡咯他尼受体与几种神经递质的膜结合受体之间不存在干扰。
