Schein Fabio Bernardo, Maia Maerle Oliveira, Witter Rute, Marcili Arlei, Camargo Lázaro Manoel de, Dutra Valéria, Nakazato Luciano, Candido Stefhano Luís, Almeida Elianara Martins de, Oliveira Anderson Castro Soares de, Pacheco Richard de Campos
Programa de Pós-graduação em Ciências Veterinárias - PPGVET, Faculdade de Medicina Veterinária - FAVET, Universidade Federal de Mato Grosso - UFMT, Cuiabá, MT, Brasil.
Faculdade de Medicine Veterinária, Universidade de Cuiabá - UNIC, Cuiabá, MT, Brasil.
Rev Bras Parasitol Vet. 2018 Oct-Dec;27(4):464-472. doi: 10.1590/S1984-296120180048. Epub 2018 Aug 30.
We evaluated the distribution of piroplasmids in equids from the Mato Grosso state in Midwestern Brazil using molecular methods and the interspecific genetic diversity. For this, 1,624 blood samples of equids from 973 farms were examined by PCR, using primer pairs that amplify a fragment of the genes rap-1 and ema-1 of Babesia caballi and Theileria equi, respectively. For molecular characterization and phylogenetic studies, 13 and 60 sequences of the rap-1 and ema-1 genes, respectively, were used to build a dendogram using maximum parsimony. B. caballi and T. equi were detected in 4.11% and 28.16% of the farms, respectively, and molecular prevalence was 2.74% for B. caballi and 25.91% for T. equi. The location of the farms and animals raised in the Pantanal ecoregion influence the probability of equids testing positive for B. caballi and T. equi . Moreover, age and herd purpose were variables significantly associated with T . equi infection. The sequences of B. caballi presented 1.95% intraspecific variability, contrasting with 2.99% in T. equi. Dendrograms for both species demonstrated the presence of subgroups with high values of support of branches. However, it is not possible to associate these groups with geographic origin and/or ecoregion.
我们使用分子方法和种间遗传多样性评估了巴西中西部马托格罗索州马属动物中梨形虫的分布情况。为此，我们采用聚合酶链式反应（PCR）对来自973个农场的1624份马属动物血液样本进行了检测，分别使用引物对扩增马巴贝斯虫（Babesia caballi）和驽巴贝斯虫（Theileria equi）的rap-1和ema-1基因片段。为了进行分子特征分析和系统发育研究，分别使用rap-1基因的13个序列和ema-1基因的60个序列，采用最大简约法构建系统发育树。在分别4.11%和28.16%的农场中检测到了马巴贝斯虫和驽巴贝斯虫，马巴贝斯虫的分子流行率为2.74%，驽巴贝斯虫为25.91%。潘塔纳尔湿地生态区饲养马属动物的地点会影响马属动物检测出马巴贝斯虫和驽巴贝斯虫呈阳性的概率。此外，年龄和畜群用途是与驽巴贝斯虫感染显著相关的变量。马巴贝斯虫的序列种内变异率为1.95%，而驽巴贝斯虫为2.99%。两个物种的系统发育树均显示存在分支支持值较高的亚组。然而，无法将这些亚组与地理起源和/或生态区联系起来。
